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Single Nanoparticle-based Dark-field Microscopic Imaging In Analytical Chemistry | 18734
ISSN: 2155-9872
Journal of Analytical & Bioanalytical Techniques
Open Access
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The light scattering of single nanoparticle, such as gold and silver nanoparticles, is stable and efficient, hence the applications
of single nanoparticle-based dark-field microscopic imaging have attracted extensive attention. Both the changes of single
nanoparticle scattering imaging color and scattering intensity can be used as effective detection signal in analytical chemistry.
In the author?s group, to study the localized surface plasmon resonance (LSPR) light scattering of single nanoparticle, first
the shape effect of single silver nanoparticle on the scattering light color and refractive index sensitivities was investigated.
It was found that particles with a large radial ratio or a tip structure always had a higher sensitivity which could be due to
the LSPR maxima at long wavelengths and the strong electric field intensity distribution. Then single nanoparticle-based
RGB analytical method was established by coding the colors of the scattering light of individual nanoparticles with the RGB
system, and the imaging date was manipulated with the IPP software. In addition, the scattering light intensity of single gold
nanoparticle was digitized and expressed as digital information through the aforementioned software. Based on the RGB
system and digitized method, it was established new scattering analytical method which had been certified in our other energy
transfer experiment. Furthermore, the single nanoparticle-based dark-field microscopic imaging was also used to monitoring
some chemical reactions. Real-time monitoring of the etching process of gold nanoparticles by iodine,
in situ
growth of single
Ag@Hg nanoalloys and photochemical reaction were successfully achieved by dark-field microscopic imaging. In addition to
the chemical reactions, the single nanoparticle-based dark-field microscopic imaging has a potential to real-time monitoring
the intracellular biochemical reactions and infection process of bacteria and virus which may provide a new method for the
diagnosis of the disease.
Biography
Chengzhi Huang has completed his PhD from Peking University in 1996 and postdoctoral studies at University of Ottawa (2000-2001) and University of Tokyo
(2001-2003). He is currently a Professor and Dean at College of Pharmaceutical Scienes at Southwest University. He is also the Director of Key Laboratory of
Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education. His research field includes utilizing light analytical science to
establish a real-time and on-line analysis method for medicament in real sample, to develop new optics- detecting technology and sensor. He has published more
than 250 papers in academic journals.
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