alexa
Reach Us +44-2476101207
Solid-Phase Extraction Of Tramadol From Plasma And Urine Samples Using A Novel Water-Compatible Molecularly Imprinted Polymer | 6107
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Solid-Phase Extraction of Tramadol from Plasma and Urine Samples Using a Novel Water-Compatible Molecularly Imprinted Polymer

International Conference & Exihibition On Analytical and Bioanalytical Techniques - 2010

Mehran Javanbakht, Abdol Mohammad Attaran, Mohammad Hadi Namjumanesh, Behrouz Akbari-adergani

ScientificTracks Abstracts: J Anal Bioanal Techniques

DOI: 10.4172/2155-9872.1000001

Abstract
In this study, a novel method is described for the determination of tramadol in biological fluids using molecularly imprinted solid-phase extraction (MISPE) as the sample clean-up technique combined with high-performance liquid chromatography (HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker, chloroform as porogen and tramadol as template molecule. The novel imprinted polymer was used as a solid-phase extraction (SPE) sorbent for the extraction of tramadol from human plasma and urine. Various parameters affecting the extraction efficiency of the polymer have been evaluated. The optimal conditions for the MIP cartridges were studied. The MIP selectivity was evaluated by checking several substances with similar molecular structures to that of tramadol. The limit of detection (LOD) and limit of quantification (LOQ) for tramadol in urine samples were 1.2 and 3.5 μg L -1 , respectively. These limits for tramadol in plasma samples were 3.0 and 8.5 μg L -1 , respectively. The recoveries for plasma and urine samples were higher than 91%.
Biography
Top