Stereoselective Determination Of Ginsenosides Rg3 And Rh2 Epimers In Plasmas By LC-MS/ MS: Application To Pharmacokinetic Studies In Various Species | 10299
Journal of Analytical & Bioanalytical Techniques
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We developed and validated an accurate and sensitive LC-MS/MS method for the simultaneous quantitation of ginsenoside
Rg3 and Rh2 epimers (R-Rg3, S-Rg3, R-Rh2, and S-Rh2) in plasma. Analytes were extracted from 0.1 mL aliquots of plasma
by liquid-liquid extraction, using 2 mL of ethyl acetate. In this assay, dioscin was used as an IS. Chromatographic separation was
conducted using an Acclaim RSLC C
column with a gradient mobile phase consisting of 0.1% formic acid in distilled water and
in acetonitrile, a flow rate of 0.35 mL/min, and a total run time of 20 min. Detection and quantification were performed using a
mass spectrometer in SRM mode with negative electrospray ionization at m/z 783.4→161.1 for R-Rg3 and S-Rg3, m/z 621.3→161.1
for R-Rh2 and S-Rh2, and m/z 867.2→761.5 for the IS. For R-Rg3 and S-Rg3, the LLoQ was 5 ng/mL, with a linear range up to
500 ng/mL; for R-Rh2 and S-Rh2, the LLoQ was 150 ng/mL, with a linear range up to 6000 ng/mL. The coefficient of variation
for assay precision was less than 10.5%, with an accuracy of 86.4?112%. The method was successfully applied to pharmacokinetic
studies after oral administration of BST204, a fermented ginseng extract, to rats, dog, and humans. In rats and humans, only S
epimers were detected in plasmas, while in dog both S and R epimers were exhibited in plasmas. This is the first report on the
simultaneous quantification of R-Rg3, S-Rg3, R-Rh2, and S-Rh2 in plasmas by LC-MS/MS.
Soo Hyeon Bae has completed her M.S. from Seoul National University, majored in pharmacokinetics. Now, she is a Ph.D. candidate in the Catholic
University of Korea and her research interest is development of LC-MS/MS assay of drugs and their metabolites in biological samples and drug
interactions with focus on pharmacokinetics. To date, she has published more than 10 papers in SCI journals.
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