Nicotinamidases are metabolic enzymes that hydrolyse nicotinamide to nicotinic acid. These enzymes are widely distributed
across biology; with examples found encoded in the genome of Mycobacteria, Archaea, Eubacteria, Protozoa, Yeast
and invertebrates, but there are none found in mammals. The catalytic mechanism is still not understood. Nicotinamidases
are required for the growth and virulence of several pathogenic microbes. These enzymes regulate life span in their respective
organisms, consistent with proposed role in the regulation of NAD
metabolism and organismal aging. The study state kinetic
parameters of nicotinamidase enzymes from
(a pathogen responsible for human
: (the pathogen that causes Lyme disease), and
(responsible for most
human malaria) are recently reported. Nicotinamidases are generally efficient catalysts. Nicotinaldehyde was determined to be a
potent competitive inhibitor of these enzymes, binding in the low micromolar to low nanomolar range for all nicotinamidases.
Inhibitions are constistent with reaction of the universally conserved catalytic Cys on each enzyme with the aldehyde functional
group to form a thiohemiacetal complexand ammonia. A catalytic mechanism that explains nicotinamidase and nicotinic acid
18O exchange chemistry for the
enzyme invoving key catalic residues, a catalytic transition metal ion, and the
intermediacy of a thioesterintermediate. In view of this, the synthesis and characterisation of 2-aminonicotinaldehyde transition
metal complexes have been taken up with their biological activity. All the complexes are characterised by different spectroscopic
study. 2-aminonicotinaldehyde and its Zn(II) complexes were characterised with single crystal X-Ray diffraction studies.
M.Rama Chary has 32 years of teaching experience for UG and PG; specialized in Inorganic chemistry. He has completed his Ph.D at the age of 35 years from Kakatiya University. He is serving as Assistant Professor at Lal Bahadur College (Re-Accredited with ?A? Grade by NAAC). He has published 8 papers in reputed journals and reviewed several books for undergraduates. His area of specialization for research is Synthesis, Characterization and Biological activity of metal complexes. He has completed 3 minor research projects sponsored by UGC, and a proposal for a major research project on NANO MATERIALS has been submitted recently to UGC.
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