Journal of Analytical & Bioanalytical Techniques
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L. (Fam. Lythraceae), or banaba, is traditionally used for diarrhea, abdominal pain, and diabetes.
Identification of banaba in the Philippine pharmacopeia employs one-dimensional thin layer chromatography (TLC) and
shows three separated components from the extracts of banaba. In this study, the aqueous and dichloromethane (DCM) fractions
of the methanolic extract of banaba were subjected to two-dimensional TLC using acetone: chloroform and n-hexane: ethyl
acetate:glacial acetic acid solvent systems. Visualization was done using iodine vapors, short- and long-wave UV light (254 nm and
365 nm), and vanillin-sulfuric-phosphoric acid spray reagent. Efficient separation was more clearly observed in the DCM fraction
chromatogram. The aqueous fraction, thereby, was subjected to reverse phase thin layer chromatography (RPTLC) wherein a 1%
acetic acid:acetonitrile (70:30) solvent system yielded the best resolution. Stability analysis using TLC in both aqueous and DCM
fractions of the extracts of leaf samples pre-exposed to UV light for 12 and 24 hours and controlled temperature of 40?C and 60?C
for three hours showed that only the increase in temperature caused a significant change in the number of spots separated and
detected. Two-dimensional RPTLC analysis or one-dimensional RPTLC analysis employing gradient elution may be applied to
the aqueous fraction to investigate if separation can further be optimized.
Banaba, Two-dimensional thin layer chromatography, Reverse phase TLC, Stability analysis.
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