Journal of Analytical & Bioanalytical Techniques
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The thin layer chromatography is the basic planar chromatographic method used for the separation of compound based on
either color reactions of the separated samples zones or on a comparison of the RF values of the analyte. Quantitative TLC
measurements are performed by densitometric scanning, with this the analytes are identified by their (corrected) RF values
and by inspection of UV/VIS spectra of the analytes and standard compounds measured in situ. If standard compounds are
not available (as in the screening of new natural agents or combinatorial chemistry samples), then it is not quantified with
densitometric scanning, the identification of unknowns has to be done with a more specific technique, such as MS detection.
The direct identification and structural characterization of the analytes on the TLC plate, through these methods are not
possible, there has been long-held interest in the development of interfaces that allow TLC to be combined with mass spectrometry
(MS), one of the most efficient analytical tools for structural elucidation. But the coupling of planar chromatography with the
mass spectrometry (TLC-MS) is difficult, but is of interest because it has a number of useful features that are not shared by other.
Sample separation from TLC is the critical process in this technique this has been done using various direct methods like
fast atom bombardment (FAB), secondary ion mass spectrometry (SIMS) and various forms of laser desorption. FAB and SIMS
probe the exposed surface of a TLC plate whereas a laser beam is able to penetrate more deeply into the absorbent layer or use of
interfaces and indirect methods like micro capillary array, sampling probes, etc.
Pornima A. Kawathekar completed M.Pharmacy from Modern College of Pharmacy, Pune University, Maharashtra, India. She has one publication
International Journal of Pharmacy.
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