Journal of Analytical & Bioanalytical Techniques
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The primary aim of this study was to evaluate and compare the efficacy and suitability of the use of beetroot juices against
nitrate rich spinach powder in supplementation studies. To carry out the analysis of nitrates and nitrites, a new extraction
method followed by ion chromatography method was developed and validated. The developed method showed higher efficiency
and better recovery. On one hand, the results from beetroot juices showed that the levels of nitrate were not only different
from what was shown on the labels but the amounts were deteriorating over time when the contents of the bottles were left
unconsumed after opening or when tested under stomach pH conditions. On other hand, the measured levels of nitrate and
nitrite in spinach powder were found to be constant over time and under stomach pH conditions with minimal deterioration.
This challenges the authenticity of the data produced by the use of beetroot juice drinks in supplementation studies. This study
further concluded that powdered forms (including spinach) are a better option as compared to juices, for two reasons: 1) the level
can be measured in the aggregated sample (1kg) so it is easier than measuring each bottle; and 2) for spinach powder, it is easier
to design a placebo control study, although lately beetroot juices are available from the companies without nitrite for this purpose
but the supply is limited to selected research groups.
Iltaf Shah has completed his Ph.D. and postdoctoral studies from Kingston University School of Pharmacy and Chemistry. He is a lecturer and
Research Scientist at Kingston University London. He has published more than 22 papers in reputed journals and scientific conferences and he
has teaching and research interest in the analysis of vitamins, drugs of abuse and antioxidants in human body matrices using HPLC, GCMS and
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