UHPLC-APPI-MS Method For High- Sensitivity And High- Throughput Analysis Of US EPA Sixteen Priority Pollutants Polynuclear Aromatic Hydrocarbons In Oyster | 6012
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

UHPLC-APPI-MS Method for High- Sensitivity and High- Throughput Analysis of US EPA Sixteen Priority Pollutants Polynuclear Aromatic Hydrocarbons in Oyster

2nd International Conference on Analytical & Bioanalytical Techniques

Sheng-Suan (Victor) Cai, Joan Stevens, Brian Nies and Jack A. Syage

ScientificTracks Abstracts: J Anal Bioanal Techniques

DOI: 10.4172/2155-9872.S1.02

In response to Gulf of Mexico deepwater horizon oil spill, we developed a QuEChERS and APPI based UHPLC-MS method for analysis of PAHs in oyster using the Agilent 1290 Infi nity LC System coupled to an Agilent 6140 Single Quadrupole LC/MS System equipped with the Syagen PhotoMate APPI source. APPI gave major ions of M + for all PAHs using chlorobenzene as a dopant. In comparison with the existing HPLC-UVD/ FLD and GC or GC-MS based methods, this method off ers better selectivity and higher sample throughput. UHPLC-APPI-MS gave 3.6 � 5.1 (Ave. 4.4) orders of dynamic linear ranges (R 2 ≥ 0.995) for these analytes. Ten grams of oyster were used for QuEChERS extraction and d-SPE cleanup. Th e method detection limits (MDLs, 6σ S/N=3) were: Naphthalene: 0.028 ppm; Acenaphthylene: 0.129 ppm; Acenaphthene: 0.102 ppm; Fluorene: 0.013 ppm; Phenanthrene: 0.040 ppm; Anthracene: 0.030 ppm; Fluoranthene: 0.030 ppm; Pyrene: 0.023 ppm; Benzo[a]anthracene: 0.030 ppm; Chrysene: 0.031 ppm; Benzo[b]fl uoranthene: 0.021 ppm; Benzo[k]fl uoranthene: 0.021 ppm; Benzo[a] pyrene: 0.027 ppm; Dibenzo[a,h]anthracene: 0.013 ppm; Benzo[g,h,i]perylene: 0.057 ppm; Indeno[1,2,3-c,d]pyrene: 0.040 ppm. Th ese MDLs were as much as four orders of magnitude lower than the US FDA levels of concern (LOCs). Th e spike recoveries ranged from 77% to 110% with %RSD of 0.6-6.7 at spike conc. (Level 1) lower or substantially lower than LOCs, and 71% to 92% with %RSD of 0.3-4.3 at Level 2 (5x Level 1).

Mr. Sheng-Suan (Victor) Cai is a Senior Applications Scientist at Syagen Technology, Inc., a wholly owned subsidiary of Morpho Detection, Inc., Safran Group. Mr. Cai has over 25 years of hands-on working experiences in the fi eld of analytical chemistry. Since joining Syagen in 2003, Mr. Cai has authored and co-authored a dozen of peer-reviewed, LC-APPI-MS(/MS) based, research journal articles and has presented over twenty APPI related presentations in scienti fi c conferences such as ASMS conference, Pittcon, Montruex LC-MS Symposium, AOCS Annual Meeting and Expo, CoSMoS, FPRW and AOAC International conference, etc.