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Ultrasensitive, Multiplexed Detection Of Infectious Disease Markers Using A Surface-enhanced Raman Scattering Immunoassay Platform | 10193
Journal of Analytical & Bioanalytical Techniques
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Early detection is central to combating infectious diseases, cancer, and bioterrorism. This presentation investigates the
development of extensible platforms and readout methodologies that address these needs by coupling gold nanoparticle
labeling and ultrasensitive detection by surface enhanced Raman scattering. Approaches will be detailed for the fabrication and
readout of chip-scale platforms that can be employed for the reliable determination of disease markers in serum and urine
matrices, probing fundamental and real-world issues for optimal performance by using tuberculosis (TB) markers as a test
vehicle. TB is the world?s second deadliest infectious disease after HIV/AIDS. The World Health Organization has called for
the development of a robust, reliable, and accurate detection method that does not suffer from the poor accuracy of current
serological-based techniques. This work will specifically examine the utility of protein arrays as an immunodiagnostic platform
designed and tested for the rapid, ultralow level measurement of various potential TB markers. Challenges related to sensitivity,
speed, and nonspecific adsorption, and fluid manipulation will also be discussed.
Marc D. Porter graduated from Wright State University (B.S. and M.S.) and The Ohio State University (Ph.D.). After a post-doctoral fellowship at
Bell Laboratories, he joined the Chemistry faculty at Iowa State University and then the Chemistry/Biochemistry faculty at Arizona State University.
He is now at the University of Utah and is the director of the Nano Institute of Utah. His laboratory works on fundamental issues and applications of
liquid-solid interfaces (e.g., nanotechnology and its applications to human and animal health). He also led an interdisciplinary team that designed a
water quality analyzer, now permanent hardware on the international space station.
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