alexa UPLCMS Method Development, Validation Of Amlodepine, Hydrochlorthiazide And Losartan In Tablet Dosage Form | 10303
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Recommended Conferences
Share This Page

UPLCMS method development, validation of amlodepine, hydrochlorthiazide and losartan in tablet dosage form

4th International Conference and Exhibition on Analytical & Bioanalytical Techniques

Anandkumar R. Tengli and Gurupadayya B. M

Accepted Abstracts: J Anal Bioanal Tech

DOI: 10.4172/2155-9872.S1.015

Abstract
A simple, sensitive and specific UPLCMS method with ELSD and PDA detection was developed for the simultaneous estimation of amlodepine, hydrochlorthiazide and losartan in tablet dosage form and telmisartan were used as an internal standard. Separation was achieved with Waters ACQUITY BEH C18, 1.7 μm, 2.1X50 mm column (Waters Corp., Ireland) with mobile phase containing acetonitrile (A) & 0.1% formic acid (B) [Gradient mode (2 min: 2% A : 98% B, 2-4 min: 24% A : 76% B, 4-5 min,50% A : 50% B)]. The target analytes were transferred into a triple quadrupole mass spectrometer equipped with an atmospheric pressure electrospray as the ionization source. The flow rate was 0.4 mLmin -1 column maintained at 25 0 C and the injection volume was 2 μl. The eluent was monitored by using ELSD and PDA detector. The selected chromatographic conditions were found to effectively separate amlodepine (AMLO), hydrochlorthiazide (HCT) and losartan (LOSAT) with retention time of 3.7, 2.5 and 3.9 min respectively. The proposed method was found to be rectilinear over the range of 25-87.5 ngmL -1 , 125-437.5 ngmL -1 and 500-1750 ngmL -1 for amlodepine, hydrochlorthiazide and losartan respectively. The proposed method was found to be accurate, precise, reproducible and specific and it can also be used for routine quality control analysis of these drugs in biological samples either alone or in combined pharmaceutical dosage forms. Keywords: UPLCMSMS, Amlodepine, Hydrochlorthiazide, Losartan, Telmisartan, Simultaneous estmation.
Biography
Anandkumar R. Tengli is perusing his Ph.D. in JSS University, JSS College of Pharmacy Mysore, under the guidance of Dr. B. M. Gurupadayya, Professor, Department of Pharmaceutical Chemistry JSS College of Pharmacy Mysore. He has published 5 papers in reputed journals
Top