UPLCMS Method Development, Validation Of Amlodepine, Hydrochlorthiazide And Losartan In Tablet Dosage Form | 10303
Journal of Analytical & Bioanalytical Techniques
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A simple, sensitive and specific UPLCMS method with ELSD and PDA detection was developed for the simultaneous
estimation of amlodepine, hydrochlorthiazide and losartan in tablet dosage form and telmisartan were used as an internal
standard. Separation was achieved with Waters ACQUITY BEH C18, 1.7 μm, 2.1X50 mm column (Waters Corp., Ireland) with
mobile phase containing acetonitrile (A) & 0.1% formic acid (B) [Gradient mode (2 min: 2% A : 98% B, 2-4 min: 24% A : 76%
B, 4-5 min,50% A : 50% B)]. The target analytes were transferred into a triple quadrupole mass spectrometer equipped with an
atmospheric pressure electrospray as the ionization source. The flow rate was 0.4 mLmin
column maintained at 25
C and the
injection volume was 2 μl. The eluent was monitored by using ELSD and PDA detector. The selected chromatographic conditions
were found to effectively separate amlodepine (AMLO), hydrochlorthiazide (HCT) and losartan (LOSAT) with retention time of
3.7, 2.5 and 3.9 min respectively. The proposed method was found to be rectilinear over the range of 25-87.5 ngmL
and 500-1750 ngmL
for amlodepine, hydrochlorthiazide and losartan respectively. The proposed method was found
to be accurate, precise, reproducible and specific and it can also be used for routine quality control analysis of these drugs in
biological samples either alone or in combined pharmaceutical dosage forms.
UPLCMSMS, Amlodepine, Hydrochlorthiazide, Losartan, Telmisartan, Simultaneous estmation.
Anandkumar R. Tengli is perusing his Ph.D. in JSS University, JSS College of Pharmacy Mysore, under the guidance of Dr. B. M. Gurupadayya,
Professor, Department of Pharmaceutical Chemistry JSS College of Pharmacy Mysore. He has published 5 papers in reputed journals
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