Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
Validation Of Bioanalytical Methods; Investigation Of Back- Conversion In Incurred Pharmacokinetic Samples | 6099
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 3732

Journal of Analytical & Bioanalytical Techniques received 3732 citations as per Google Scholar report

Journal of Analytical & Bioanalytical Techniques peer review process verified at publons
Indexed In
  • CAS Source Index (CASSI)
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Academic Journals Database
  • Open J Gate
  • Genamics JournalSeek
  • JournalTOCs
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Ulrich's Periodicals Directory
  • Electronic Journals Library
  • RefSeek
  • Directory of Research Journal Indexing (DRJI)
  • Hamdard University
  • OCLC- WorldCat
  • Scholarsteer
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Euro Pub
Share This Page

Validation of Bioanalytical Methods; Investigation of Back- Conversion in Incurred Pharmacokinetic Samples

International Conference & Exihibition On Analytical and Bioanalytical Techniques - 2010

Luigi Silvestro, Simona Rizea Savu, Silviu Savu Ariana Tudoroniu, Adriana Iordachescu, Adelina Moise

ScientificTracks Abstracts: J Anal Bioanal Techniques

DOI: 10.4172/2155-9872.1000001

Introduction: Lately growing attention has been dedicated to the occurrence of back-conversion, intended as the transformation of a drug metabolite to the parent compound during samples handling. Back-conversion brings to inaccurate quantitative determinations and EMA is imposing the validation of back-conversion in analytical methods for bioequivalence (Guideline on Bioequivalence 2010). While it is clear now that validation procedures shall check back-conversion, other methods than the use of biological samples spiked with metabolite standards, are unavailable. Unfortunately the metabolism of several well-known drugs is unclear and new molecules are coming: what to do? A validation approach based on incurred samples of subjects treated with studied drugs was developed; the results collected on 20 drugs will be presented. Materials and methods: In all validations, HPLC-MS/MS and reversed phase or ion exchange columns separations were employed. These data compare the evaluation of back-conversion by classical procedures (spiked samples) with the findings gathered using incurred samples analyzed soon after sampling, then after different period/conditions of storage. Results and conclusions: With one drug, only the incurred samples permitted to validate back-conversion and with another-one they allowed to perform a validation when metabolite standards were unavailable. In all other cases both approaches gave similar results; it never occurred that incurred samples results were contradictory with those based on spiked samples. In conclusion the incurred samples approach is very useful, if not the only one in some cases, to adequately validate analytical methods for back-conversion.