ZSM-5 Based Chromatographic Adsorbents As A Potential Tool For Biomarkers Detection | 10224
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
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ZSM-5 based chromatographic adsorbents as a potential tool for biomarkers detection

4th International Conference and Exhibition on Analytical & Bioanalytical Techniques

Shigemi Goda, Roman Selyanchyn and Seung-Woo Lee

Posters: J Anal Bioanal Tech

DOI: 10.4172/2155-9872.S1.014

Preliminary research that have been conducted and numerous works published by other research groups confirm the existence of small molecular cancer biomarkers (SMCBs). However, the origin of these metabolites (that can serve as clinical disease markers) still remains unknown. Clear understanding of how and where SMCBs are generated in a human organism is of urgent importance. Novel adsorptive materials with polymer mixed with zeolites are under investigation and give promising results due to improvement of adsorptive coatings. For instance, PDMS/zeolite membranes, rarely reported for filtration purpose, to the best of our knowledge, in our current study are used for the first time as adsorbents for preconcentration and extraction of volatile and semivolatile compounds from complex matrices for further GC-MS analysis. As found in preliminary study, composite of zeolites with polymer base (Tenax TA, PDMS) possess higher adsorption capacity, better retention of the adsorbed compounds, enhanced diffusion and respectively equilibration times. Multi compound test of PDMS doped with 10% ZSM-5 shown that addition of zeolite extends not only improves the adsorption capacity of PDMS but spectra of adsorbed analytes (+highly volatile, +hydrophilic) that are not usually adsorbed or effectively retained in PDMS alone. Current work will describe process of the investigation of cancer cell cultures in-vitro . Using thin-film microextraction (TFME) coupled with a gas chromatography mass spectrometry (GC-MS) analytical method, we are conducting the search of SMCBs generated in-vitro in the several types of cell cultures.
Shigemi Goda has completed her B.Sc. at the age of 22 years from the University of Kitakyushu and now she is the master student in the same University. Her research interests include the detection of small molecular markers of cancer from the in-vitro samples.