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A Method for Screening Sensors of Calcium Signaling in Rice
Zhiming Yu, Yue He and Jun Ni*
College of Life and Environmental Sciences, Hangzhou Normal University, China
- Corresponding Author:
- Ni J
College of Life and Environmental Sciences
Hangzhou Normal University
Hangzhou, 310018, China
Tel: 86-571-28868542
E-mail: nijun@hznu.edu.cn
Received Date; May 24, 2015; Accepted Date; June 30, 2015; Published Date; July 04, 2015
Citation: Yu Z, He Y, Ni J (2015) A Method for Screening Sensors of Calcium Signaling in Rice. Biosens J 4:116. doi:10.4172/2090-4967.1000116
Copyright: © 2015 Yu Z, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Although rice is an important staple food crop of nearly half of the world population, the discoveries in calcium ion signal were mainly focused on the model plant Arabidopsis and researches in rice are very superficial and no calcium ion sensors have been found yet. Recently, the recombinant aequorin was introduced into rice, and the Ca2+ responses to NaCl and H2O2 in rice roots were characterized. This system can also be used to identify novel Ca2+-coupled stress sensors or pathways after the establishment of an EMS-mutagenized population. In this paper, we present the screening design of the specific program in details.
