A Novel Electrophoretic Deposition Device: Effects of Alginate Viscosity Grade on Deposition KineticsChris R Jackson, Pavan MV Raja and Satya Prakash*
Biomedical Technology and Cell Therapy Research Laboratory, Departments of Biomedical Engineering, Physiology, and Artificial Cells and Organs Research Center, Faculty of Medicine, McGill University, Duff Medical Building, 3775 University Street, Montreal, QC H3A 2B4, Canada
- Corresponding Author:
- Satya Prakash
Biomedical Technology and Cell Therapy Research Laboratory
Departments of Biomedical Engineering, Physiology
and Artificial Cells and Organs Research Center
Faculty of Medicine, McGill University, Duff Medical Building
3775 University Street, Montreal, QC H3A 2B4, Canada
E-mail: [email protected]
Received date: February 02, 2012; Accepted date: February 27, 2012; Published date: February 29, 2012
Citation: Jackson CR, Raja PMV, Prakash S (2012) A Novel Electrophoretic Deposition Device: Effects of Alginate Viscosity Grade on Deposition Kinetics. J Biotechnol Biomaterial S6:002. doi:10.4172/2155-952X.S6-002
Copyright: © 2012 Jackson CR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A novel device was designed to perform electrophoretic deposition under tightly controlled conditions. The device physical parameters were investigated by depositing three different viscosity grades of sodium alginate hydrogels. A thin metallic rectangular substrate was used to obtain the various time dependant deposition rates of the gels. The resulting deposition curves showed the effective electrophoretic mobilities of the low, medium and high viscosity grade gels were 0.0610 cm2/Vs, 0.0584 cm2/Vs and 0.0909 cm2/V sand that the ratios of gel deposit to solution resistivities were 21.0, 16.2 and 47.5 respectively. Following electrophoretic deposition, the gels were cross-linked in a 0.1 M CaCl2 solution in order to further solidify the gels. Cross-linking reduced the masses of the gels to 50.9 ± 1.8%, 26.7 ± 2.0%, and 28.5 ± 1.3% of their initial masses respectively. Lyophilization was applied to the gels to determine the alginate content of the gels. Immediately after deposition the alginate mass fractions of the low, medium and high viscosity grade gels were 2.92 ± 0.49%, 2.70 ± 0.08% and 2.94 ± 0.15% respectively. Cross-linking caused the mass fraction of alginate to increase significantly to 5.59 ± 0.07%, 7.11 ± 0.37% and 7.02 ± 0.24% respectively. The device in this study provided sufficient data to model the electrophoretic deposition rates. The technique can be expanded to other hydrogel species which can be used in a variety of biomedical and biotechnological applications.