A Phenomenon found in the Transcriptional Regulation Study on Mouse Melanocortin 3 ReceptorÃ¢Â€Â”Link the Transcriptional Activity with the Phosphorylation and Glycosylation of Transcription Factors
|Wen X1, Zeng WW1* and Jack A Yanovski2|
|1Developmental Neurobiology Section, NHLBI, NIH, USA|
|2UGO, Eunice Kennedy Shriver, NICHD, NIH, USA|
|Corresponding Author :||Zeng WW
Developmental Neurobiology Section
NHLBI, NIH, USA
Tel: +1 301 496 2651
Fax: +1 301 402 0574
|Received November 26, 2013; Accepted December 17, 2013; Published December 19, 2013|
|Citation: Wen X, Zeng WW, Yanovski JA (2013) A Phenomenon found in the Transcriptional Regulation Study on Mouse Melanocortin 3 Receptor—Link the Transcriptional Activity with the Phosphorylation and Glycosylation of Transcription Factors. J Obes Weight Loss Ther 3:202.doi:10.4172/2165-7904.1000202|
|Copyright: © 2013 Wen X, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
Melanocortin 3 receptor (MC3R) is involved in the regulation of energy homeostasis. Study on mouse MC3R promoter has been performed following the determination of its transcription starting site, mouse MC3r deletion constructs were built by sub cloning into pGL3 vector, then the gene reporter assay of the seven deletion constructs was tested by Dual Luciferase Assay.
On the other hand, phosphorylation and glycosylation play an important role in the transcription regulation. The global distribution of phosphorylation and glycosylation on transcription factor could be found in open source database.
In this report, relative luciferase activity and the distribution of phosphorylation-glycosylation sites on promoter model transcription factor were plotted, for the first time, on the same chart, within a 3.2 kb upstream the methinoin ATG, the two trend lines of the relative luciferase activity and the distribution of phophorylation-glycosylation sites, they were found tend to be negative reciprocal.