A Point of Care Real Time PCR Platform Based on Silicon Technology
Salvatore Petralia, Eloisa Castagna M, Massimo O Spata, Maria Grazia Amore and Sabrina Conoci*
Sabrina Conoci, STMicroelectronics, Stradale Primosole 50, Catania 95121, Italy
- *Corresponding Author:
- Sabrina Conoci, STMicroelectronics
- Stradale Primosole
50, Catania 95121, Italy
- Tel: 41 227371000
- E-mail: [email protected]
Received Date: February 26, 2016; Accepted Date: April 04, 2016; Published Date:April 10, 2016
Citation: Petralia S, Eloisa Castagna M, Spata MO, Amore MG, Conoci S (2016) A Point of Care Real Time PCR Platform Based on Silicon Technology. Biosens J 5:136. doi: 10.4172/2090-4967.1000136.
Copyright: © 2016 Petralia S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The miniaturization of a real time PCR amplification systems are key to genetic point-of-care (POC) diagnostics to offer sample-in answer-out analysis. Actually, miniaturization typically enables shorter analysis times, reduces reagent consumption, minimizes risk of sample contamination, and often enhances assay performance. Here we present the genetic real time PCR PoC system based on silicon microchip able to address the quantitative and qualitative identification of multiple analytes of specific nucleic acids (DNA, RNA) sequences. Thanks to the combination of integrated silicon temperature sensors and heaters, the specific chip design architecture and the smart detection software, an improvement of sensitivity is achieved respect to the commercial tools.