Beneficial Effect of a Multifunctional Polyphytocompound in Experimental Prostatic Hyperplasia in RatsMakoto Kantah1, Birbal Singh2, Hala Sweed3, Geraldo Balieiro Neto4,5, Nikhil Kumar6, Fernando Bahdur Chueire4, Francesco Marotta1*, Aldo Lorenzetti1, Richard Bellow1 and Umberto Solimene7
- *Corresponding Author:
- Francesco Marotta
ReGenera Research Group for Aging Intervention and San Babila Clinic
Corso Matteotti, 1/A 20121 Milano, Italy
E-mail: [email protected]
Received Date: February 27, 2017; Accepted Date: March 24, 2017; Published Date: March 27, 2017
Citation: Kantah M, Singh B, Sweed H, Neto GB, Kumar N, et al. (2017) Beneficial Effect of a Multifunctional Polyphytocompound in Experimental Prostatic Hyperplasia in Rats. Clin Pharmacol Biopharm 6:169. doi: 10.4172/2167-065X.1000169
Copyright: © 2017 Kantah M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The aim of the present study was to assess the efficacy of a poly-phytocompound in a model of experimental BPH. Adult 8 weeks male Wistar rats were subjected to complete orchiectomy under anesthesia (i.p. injection of 100 mg/kg body weight of sodium pentobarbital). After castration, experimental BPH was reproduced by subcutaneous injection of testosterone (20 mg/kg) for 4 weeks and, at the same time, rats randomly divided in 3 groups (15 rats each): (A) untreated BPH model; (B) BPH plus TR10/P3795 orally and (C) BPH plus finasteride (10 mg/kg body weight) administered orally as positive control group. A third group (D) of sham-operated rats served as control. Both TR10/P3795- and finasteride-treated groups showed a significant (p<0.05) and comparable reduction of all morphometric parameters (volume, weight and weight/body weight ration) which were grossly abnormal in untreated BPH model (p<0.01 vs. sham-op.). Moreover, both treatment schedule maintained a near-to-normal 3 h urinary output (p<0.01 vs. untreated BPH). Untreated BPH showed a significant increase of epithelial size and thickness and these features were equally decreased by TR10/P3795 and finasteride (p<0.05). Either TR10/P3795 or finasteride brought about a significant decrease of serum level of DHT and PAP (p<0.05 vs. sham). There was no difference among the two treatments. Prostatic tissue concentration of MDA, IL-6, TNFα and TGFβ1 significantly increased in untreated BPH model (p<0.001). All these parameters significantly decreased, although not normalised, in TR10/P3795-treated group (p<0.05 vs. sham and vs. finasteride). Finasteride determined only a not significant trend decrease of IL-6 and TNFα. Given the multifactorial aetiology of BPH, the data from this experimental model show the promising larger spectrum of mechanisms of action of the tested poly-phytocompound.