Blocking Non-Specific Binding for Phage-Based Magnetoelastic Biosensors
- Corresponding Author:
- Hu J
School of Materials Science and Engineering
Changzhou 213164, China
E-mail: [email protected]
Received Date: September 04, 2015; Accepted Date: October 31, 2015; Published Date: November 02, 2015
Citation: Hu JJ, Chai Y, Horikawa S, Chin BA, Hu J (2015) Blocking Non-Specific Binding for Phage-Based Magnetoelastic Biosensors. Biosens J 4:130.doi:10.4172/2090-4967.1000130
Copyright: © 2015 Hu JJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The magnetoelastic (ME) biosensors are used to detect pathogen in fresh juice or milk by solenoid coil, and also developed for real-time, direct pathogen detection on food surfaces by surface-scanning coil. This paper presents blocking effect of different reagents on non-specific binding for detecting Salmonella typhimurium in apple juice using phage-based magnetoelastic biosensors. Three different blocking reagents of Bovine serum albumin, Superblock blocking buffer and blocker BLOTTO were used and evaluated. The results shows that blocker BLOTTO has the best blocking effect on non-specific binding.