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Chemical Synthesis, Characterization and Bioactivity Evaluation of Hydroxyapatite Prepared from Garden snail (Helix aspersa) | OMICS International | Abstract
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
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Research Article

Chemical Synthesis, Characterization and Bioactivity Evaluation of Hydroxyapatite Prepared from Garden snail (Helix aspersa)

Anjuvan Singh1* and K. M. Purohit2
1Department of Biotechnology, Lovely School of Sciences, Lovely Professional University, Phagwara, India
2Department of Chemistry, National Institute of Technology, Rourkela –769008, Orissa, India
Corresponding Author : A. Singh
Lecturer, Department of Biotechnology
Lovely School of Sciences Lovely Professional University
Phagwara, INDIA
Tel: +91-7837651937
E-mail: [email protected]
Received March 31, 2011; Accepted April 28, 2011; Published May 06, 2011
Citation: Singh A, Purohit KM (2011) Chemical Synthesis, Characterization and Bioactivity Evaluation of Hydroxyapatite Prepared from Garden snail (Helix aspersa). J Biotechnol Biomaterial 1:104. doi: 10.4172/2155-952X.1000104
Copyright: © 2011 Singh A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Early years, bio-nano-composites materials are interesting in advanced engineering applications fields. In order to, biological properties of the 10Ce-TZP/Al2O3 nano-composite was evaluated by the addition of HA nanopowder for medical applications. In this research, a bio-nano-composite consisting of CeO2- stabilized zirconia (Ce-TZP), Al2O3 and Hydroxyapatite (HA) powders proposed to use for medical application .The first step, nano-powders of Ce-TZP and Al2O3, were blended by fast milling machine. Then, nano-powder of HA was added to the alumina - zirconia (ZA) mixture from 10 to 40 vol%, and homogenized again. In final step, mixed powder was cold pressed and sintered at1600°C for 120 min. Crystal phase of the sintered samples were characterized by X-ray diffraction(XRD),the density and bending strength were measured and cellular response test was performed with osteoblastic cell-lines by MG63 cell-lines and the morphology of the proliferated cells was observed with FESEM too. From the strength and cellular response evaluations of samples, specimens with 30 vol% HA showed the best result, also XRD patterns confirmed this results.

Keywords

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