alexa

GET THE APP

Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
Design of Bioluminescence Sensor System for Rapid Detection of Bacteria in Samples without Cultivation | OMICS International | Abstract
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Design of Bioluminescence Sensor System for Rapid Detection of Bacteria in Samples without Cultivation

Saroj Hole1* and Dhote DS2

1Assistant professor of modern college, Pune University, Maharashtra, India

2Reader, Amravati University, Tapovan, Amravati, Maharashtra, India

Corresponding Author:
Saroj Hole
Assistant professor of modern college
Pune University, Maharashtra, India
Tel: 9850018668
E-mail: [email protected]

Received date: August 08, 2011; Accepted date: December 12, 2011; Published date: December 14, 2011

Citation: Saroj H, Dhote DS (2011) Design of Bioluminescence Sensor System for Rapid Detection of Bacteria in Samples without Cultivation. J Biotechnol Biomaterial 1:118. doi:10.4172/2155-952X.1000118

Copyright: © 2011 Saroj H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Based on the principle of ATP bioluminescent reaction, we design a portable bioluminescence sensor system to detect bacteria in samples rapidly, which included an optical sensing cell, a flow injection unit and a center processing unit. A fast ATP extraction technique was developed by using trichloro acetic acid as extraction reagent Experimental results showed that the intracellular ATP could be released from bacterial cells sufficiently within 5 min by TCA In the presence of Lucifer ace the reaction between ATP and lucifer5in would generate light and the luminescence intensity RLU was proportional to the amount of bacteria existed in the sample. The dynamic range of the sensor system is 10-107 CFU /ml. The linear regression coefficient was 0.976, and the detection limit was 10CFU /ml. The whole testing time needed was about 10 min. Compared with the plate count method, bacteria concentration in samples could be detected rapidly by using the portable sensor system without cultivation.

Keywords

Google Scholar citation report
Citations : 1820

Journal of Biotechnology & Biomaterials received 1820 citations as per Google Scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • EBSCO A-Z
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Share This Page
https://milanobet.fun/ https://mobilbahis.fun/ https://oleybet.fun/ https://onbahis.fun/ https://padisahbet.fun/ https://piabetgiris.xyz/ https://pusulabet.fun/ https://pulibet.fun/ https://restbet.fun/ https://satbet.fun/ https://1xbetx.fun/ https://asyabahisx.xyz/
Top