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ISSN: 2155-9910

Journal of Marine Science: Research & Development
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Research Article

Determining and Estimation of Antibody Production in the Bubble Eye, Goldfish

Natsuki Nukada1, Eriko Avsar-Ban1, and Yutaka Tamaru1,2,3*

1Department of Life Sciences, Graduate School of Bioresources, Mie University, 1577 Kurimamachiya, Tsu, Mie 514-8507, Japan

2Department of Bioinfomatics, Mie University Advanced Science Research Centre, Mie University, 1577 Kurimamachiya, Tsu, Mie 514-8507, Japan

3Laboratory of Applied Biotechnology, Mie University Industrial Technology Innovation Institute, Mie University, 1577 Kurimamachiya, Tsu, Mie 514-8507, Japan

*Corresponding Author:
Yutaka Tamaru
Graduate School of Bioresources
Mie University
1577 Kurimamachiya, Tsu
Mie 514-8507, Japan
Tel: +81 59 231 9560
Email: [email protected]

Received date: December 05, 2016; Accepted date: December 22, 2016; Published date: December 28, 2016

Citation: Nukada N, Avsar-Ban E, Tamaru Y (2016) Determining and Estimation of Antibody Production in the Bubble Eye, Goldfish. J Marine Sci Res Dev 6:219. doi:10.4172/2155-9910.1000219

Copyright: © 2016 Nukada N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Development of antibody production technologies is necessary for diagnostic treatments and drug discovery. In general, mammals are used as host animals to produce antigen-specific antibody. However, such host animals have never produced several specific antibodies because host animals may not recognize foreign proteins. To overcome this problem, we used teleost to produce antibodies because teleost are evolutionally localized in the origin of vertebrates and have an acquired immune system in addition to the innate immune system. In particular, we attempt to produce antibody using “Bubble Eye” as a kind of goldfish (Carassius auratus), which has sacs filled with lymph liquid, as an immune animal. In this study, a recombinant EGFP-His was expressed in E. coli and then injected into Bubble Eye’s sac in every two weeks. The antibodies were collected from sac instead of blood. Furthermore, a sandwich dot blotting was developed for detection of antibodies against EGFP-His. The antigen-specific antibodies were detected after 42 days from first immunization.

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