Development of a Separation and Detection System for Bacillus anthracis Spores Based on Peptide Conjugates Identified from Peptide Library
3Department of Chemical and Biomolecular Engineering, Department of Bio and Brain Engineering, Department of Biological Sciences, BioProcess Engineering Research Center, Bioinformatics Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST, 291 Gwahak-ro, Yuseonggu, Daejeon 305-701, Republic of Korea
- Corresponding Author:
- Tae Jung Park
Department of Chemistry, Chung-Ang University
84 Heukseok-ro, Dongjak-gu, Seoul 156-756, Republic of Korea
E-mail: [email protected]
Received Date: November 18, 2014; Accepted Date: December 29, 2014; Published Date: January 05, 2015
Citation: Kim GW, Park JP, Lee SY, Park TJ (2015) Development of a Separation and Detection System for Bacillus anthracis Spores Based on Peptide Conjugates Identified from Peptide Library. Biosens J 4:112. doi:10.4172/2090-4967.1000112
Copyright: © 2015 Kim GW, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A simple, specific and rapid identification system against Bacillus anthracis spores was developed using specific capture peptides conjugated with a bead-based biosensor, which was tightly and specifically bound onto the spore surface of B. anthracis. We successfully detected and separated B. anthracis spores from the spores of B. thuringiensis and B. cereus using this peptide-magnetic bead conjugates by fluorescence and anthrax-specific analyses. For more convenient mediation of high-throughput detection against B. anthracis spores, streptavidin-biotin interactions of spore-peptide and spore-peptide-magnetic bead conjugates were performed, and we demonstrated the separation and detection of B. anthracis spores using this method. In the presence of mixed condition of several types of Bacillus spores, the B. anthracis spores were easily separated using the oligopeptide-conjugated magnetic beads, thus allowing the clear detection of B. anthracis spores from B. cereus, B. subtilis, and B. thuringiensis spores. This oligopeptidebased strategy was rapidly and unambiguously identified as little as one viable B. anthracis spore in less than 1 h with a simple binding assay format. When assessed for its effectiveness for specifically and selectively detection of environmental spores phylogenetically similar to B. anthracis spores, such as B. thuringiensis and B. cereus spores, the system was free of false-positive signals.