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Dissecting Signaling Pathways by the Use of Genetically-Encoded Biosensors: Dynamic Matters
Pauline Vandame1, Davel Trinel2, Jean-François Bodart1* and Corentin Spriet2
1University Lille, Régulation des Signaux de Division Team, UMR 8576 CNRS, F-59000 Lille, France
2University Lille, TISBio, UMR 8576 CNRS, F-59000 Lille, France
- *Corresponding Author:
- Jean-François Bodart
University Lille, Régulation des Signaux de Division Team
UMR 8576 CNRS
F-59000 Lille, France
Tel: 330320436867
E-mail: Jean-Francois.Bodart@univ-lille1.fr
Received date: February 2, 2016; Accepted date: May 12, 2016; Published date: May 18, 2016
Citation: Vandame P, Trinel D, Bodart JF, Spriet C (2016) Dissecting Signaling Pathways by the Use of Genetically-Encoded Biosensors: Dynamic Matters. Biosens J 5: 1000138. doi:10.4172/2090-4967.1000138
Copyright: © 2016 Vandame P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Among biosensors, genetically-encoded FRET biosensors raised hope to dissect signaling pathways by monitoring enzymatic activities or second messengers levels in living cells and even in living and developing organisms. FRET (Froster resonance Energy Transfer) is a radiationless energy transfer from donor to acceptor fluorophore. For most genetically encoded donor/acceptor pair, this transfer can only occur if they are separated by a distance less than ~10 nm. A FRET based sensor is made up of an adapted bioreceptor tagged on both ends with appropriate fluorophores.
