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Hindi Research Article
Effect of Polygonum persicaria Agglutinin on Digestive α-amylase of Rice Striped Stem Borer
| Arash Zibaee* | ||
| Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| Corresponding Author : | Arash Zibaee Department of Plant Protection Faculty of Agricultural Sciences University of Guilan, Rasht, Iran Tel: 0911-237-9585 Fax: 0131-669-0281 E-mail: arash.zibaee@gmx.com, arash.zibaee@guilan.ac.ir |
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| Received January 06, 2014; Accepted January 28, 2014; Published January 30, 2014 | ||
| Citation: Zibaee A (2014) Effect of Polygonum persicaria Agglutinin on Digestive α-amylase of Rice Striped Stem Borer. J Rice Res 2:120. doi: 10.4172/jrr.1000120 | ||
| Copyright: © 2014 Zibaee A. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | ||
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Abstract
Results of the current study revealed that a lectin extracted from Polygonum persicaria L. had significant inhibition on digestive α-amylase of Chilo suppressalis Walker. Concentrations 0.05 to 2 mg/ml of PPA significantly decreased enzymatic activity from 5 to 72%. The highest inhibition of α-amylase was obtained at pH 9 while the optimal pH for amylolytic activity was found to be 8 and 9. Besides, optimal temperature of α-amylase activity was at 30°C but the highest inhibition of the enzyme was found at temperatures of 30-40°C. Kinetic parameters of α-amylase revealed lower and higher amounts of Vmax and Km in PPA treated enzyme indicating mixed inhibition. In vitro inhibition of α-amylase clearly showed that PPA could intervene in digestive process of C. suppressalis and decrease nutrient income of larvae. Further experiments are required to fully understand negative effects of PPA against rice striped stem borer to reach an efficient pest control via providing resistant varieties.
