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Effect of <em>Polygonum persicaria</em> Agglutinin on Digestive andalpha;-amylase of Rice Striped Stem Borer | OMICS International | Abstract
ISSN: 2375-4338

Rice Research: Open Access
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Research Article

Effect of Polygonum persicaria Agglutinin on Digestive α-amylase of Rice Striped Stem Borer

Arash Zibaee*
Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
Corresponding Author : Arash Zibaee
Department of Plant Protection
Faculty of Agricultural Sciences
University of Guilan, Rasht, Iran
Tel: 0911-237-9585
Fax: 0131-669-0281
E-mail: [email protected], [email protected]
Received January 06, 2014; Accepted January 28, 2014; Published January 30, 2014
Citation: Zibaee A (2014) Effect of Polygonum persicaria Agglutinin on Digestive α-amylase of Rice Striped Stem Borer. J Rice Res 2:120. doi: 10.4172/jrr.1000120
Copyright: © 2014 Zibaee A. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Results of the current study revealed that a lectin extracted from Polygonum persicaria L. had significant inhibition on digestive α-amylase of Chilo suppressalis Walker. Concentrations 0.05 to 2 mg/ml of PPA significantly decreased enzymatic activity from 5 to 72%. The highest inhibition of α-amylase was obtained at pH 9 while the optimal pH for amylolytic activity was found to be 8 and 9. Besides, optimal temperature of α-amylase activity was at 30°C but the highest inhibition of the enzyme was found at temperatures of 30-40°C. Kinetic parameters of α-amylase revealed lower and higher amounts of Vmax and Km in PPA treated enzyme indicating mixed inhibition. In vitro inhibition of α-amylase clearly showed that PPA could intervene in digestive process of C. suppressalis and decrease nutrient income of larvae. Further experiments are required to fully understand negative effects of PPA against rice striped stem borer to reach an efficient pest control via providing resistant varieties.

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