alexa Genetic Diversity Analysis of Urtica Parviflora in Utta
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
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Research Article

Genetic Diversity Analysis of Urtica Parviflora in Uttarakhand Himalayas by Rapid Marker

Sandeep Sharma1*, Syed Atif Ali2, Alok Khare3 and Tapan Kumar Nailwal2

1National Bureau of Plant Genetic Resources (ICAR), Pusa Campus New Delhi, India

2Departments of Biotechnology, Kumaun University, Nainital, Uttarakhand, India

3Departments of Botany, Bareilly College, Bareilly, India

Corresponding Author:
Sandeep Sharma
Division of Plant Physiology
IARI New Delhi 110012, India
Tel: +919557285687
E-mail: [email protected]

Received date: January 30, 2015; Accepted date: May 25, 2015; Published date: June 01, 2015

Citation: Sharma S, Ali SA, Khare A, Nailwal TK (2015) Genetic Diversity Analysis of Urtica Parviflora in Uttarakhand Himalayas by Rapid Marker. J Biotechnol Biomater 5:183. doi:10.4172/2155-952X.1000183

Copyright: © 2015 Sharma S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Urtica parviflora is herbaceous perennial medicinal plant distributed in Himalayan region from 400m to 3000m amsl altitude range. In the present study, five genotypes of U. parviflora were collected from different altitudes having variability in morphological traits. This genotype was subjected to Random Amplified Polymorphic DNA (RAPD) marker analysis to assess the genetic diversity at molecular level and develop molecular marker for their identification. Fairly rich genetic diversity was found through RAPD marker analysis. A total 66 scorable bands were produced in five samples with 8 primers. Out of 66 bands, 43 bands were found to be polymorphic i.e., 65.15% polymorphism. The dendrogram of samples shows three major clusters. The samples of similar altitudes were found to be present in one cluster.


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