Growth Kinetics of Acclimated Mixed Culture for Degradation of Isopropyl Alcohol (IPA) | OMICS International | Abstract
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

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Research Article

Growth Kinetics of Acclimated Mixed Culture for Degradation of Isopropyl Alcohol (IPA)

Smita Raghuvanshi1*, Suresh Gupta1 and B. V. Babu2

1Assistant Professor, Department of Chemical Engineering, Birla Institute of Technology and Science (BITS), PILANI–333 031, Rajasthan, India

2Director, Institute of Engineering and Technology (IET), JK Lakshmipat University (JKLU) JAIPUR–302026, Rajasthan, India

Corresponding Author:
Smita Raghuvanshi
Department of Chemical Engineering
Birla Institute of Technology and Science (BITS)
PILANI–333 031 (Rajasthan) India
Tel: +91-1596-245073 ext. 215
Fax: +91-1596-244183
E-mail: [email protected]; [email protected]

Received date: November 06, 2012; Accepted date: November 29, 2012; Published date: December 03, 2012

Citation: Raghuvanshi S, Gupta S, Babu BV (2012) Growth Kinetics of Acclimated Mixed Culture for Degradation of Isopropyl Alcohol (IPA). J Biotechnol Biomaterial S13:002. doi:10.4172/2155-952X.S13-002

Copyright: © 2012 Raghuvanshi S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Iso-propyl alcohol (IPA) is an organic chemical regarded as a potential pollutant. The permissible exposure limit (PEL) of IPA specified by Occupational Safety and Health Administration (OSHA) is 400 mg L-1. In this study the aerobic biodegradation of IPA is carried out by an acclimated mixed culture obtained by sewage treatment plant, for the range of 200-700 mg L-1 of initial IPA concentration. The batch degradation study demonstrated that the maximum growth rate obtainable is 0.337 h-1. To explain the inhibition effects, different kinetic growth models such as Haldane, Luong and Edward models are applied. The experimental data are found to fit well with inhibition models having the values of coefficient of determination (R2) of 0.989, 0.986 and 0.984 respectively for Haldane, Luong and Edward models. Based on the disappearance of IPA, degradation is modeled by the three-half-order kinetics and the resulting kinetic parameters are reported.