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Hindi Research Article
Hybrid Amniotic Membrane Dressing with Green Silver Nanoparticles as Bioengineered Skin for Wounds and Burns: A Pilot Studies
Balasundari Ramesh1*, Jaikanth Chandrasekaran2, Sangeetha Jeevankumar2, George Jacob1and Kotturathu Mammen Cherian21Apollo Cancer Speciality Hospital, Teynampet, Chennai, 600035, India
2Frontier Lifeline Pvt. Ltd., R80C, Ambattur Industrial Estate Road, Mugappair, Chennai 600101, India
- *Corresponding Author:
- Ramesh B
Department of Regenerative Medicine
Apollo Cancer Speciality Hospital, Teynampet
Chennai, India 600035
Tel: 09036464769
E-mail: drbalasundari_r@apollohospitals.com
Received date: July 06, 2017; Accepted date: September 21, 2017; Published date: September 28, 2017
Citation: Ramesh B, Chandrasekaran J, Jeevankumar S, Jacob G, Cherian KM (2017) Hybrid Amniotic Membrane Dressing with Green Silver Nanoparticles as Bioengineered Skin for Wounds and Burns: A Pilot Studies. J Biotechnol Biomater 7:272. doi:10.4172/2155-952X.1000272
Copyright: © 2017 Ramesh B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Abstract
Fabrication of 3D novel scaffolds is very challenging and critical to achieve the appropriate function for tissue regeneration. This study aims to develop a hybrid biological nano-scaffold using Liquid nitrogen (LN2) decellularised human amniotic membrane (hAM), human umbilical cord derived collagen nano-fibres and green silver nanoparticles obtained by green synthesis of curcumin. A novel attempt was made to develop in vitro bioengineered dermal layer by seeding the scaffold with cord blood derived Mesenchymal stem cells (CBMSCs) and trans-differentiating them to keratinocytes and dermal fibroblast like cells using keloid foreskin conditioned media. Furthermore, the small animal experiments in Albino Wistar rats prove it to be a safe healing product without scar formation. This novel scaffold can be a promising dressing material for none healing ulcers and burns. This scaffold has long shelf life, easy to apply, have sustained/controlled release of silver, the minimum frequency of dressing changes, manage excessive exudates, moisture retainment, reduce inflammation and facilitate autolytic debridement.
