alexa Identification of an Alpha-Tubulin Gene from the Chines
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
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Research Article

Identification of an Alpha-Tubulin Gene from the Chinese Mitten Crab Eriocheir sinensis: Expression Profiles under Immune Challenge and during Larval Development, Autoimmune hepatitis, Immune Cell Therapy, Auto immune Disease

Peng Li*, Xianfeng Jiang, Bin Sun, Yubo Lin, Jie Yan, Kaiya Zhou*

Jiangsu Key Laboratory for Biodiversity and Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China

Corresponding Author:
Peng Li
Jiangsu Key Laboratory for Biodiversity and Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases
College of Life Sciences, Nanjing Normal University, Nanjing 210023, China
Tel: +86-25-85891667
Fax: +86-25-85891786
E-mail: [email protected]

Kaiya Zhou
Jiangsu Key Laboratory for Biodiversity and Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases
College of Life Sciences, Nanjing Normal University, Nanjing 210023, China
Tel: +86-25-85891667
Fax: +86-25-85891786
E-mail: [email protected]

Received date: August 31, 2015; Accepted date: September 23, 2015; Published date: September 30, 2015

Citation: Li P, Jiang X, Sun B, Lin Y, Yan J, et al. (2015) Identification of an Alpha- Tubulin Gene from the Chinese Mitten Crab Eriocheir sinensis: Expression Profiles under Immune Challenge and during Larval Development. J Biotechnol Biomater 5:201. doi:10.4172/2155-952X.1000201

Copyright: © 2015 Li P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

In this study, an alpha-tubulin gene (EsTUBA, GenBank: KJ509188) was isolated and identified from the Chinese mitten crab Eriocheir sinensis. The EsTUBA mRNA expression patterns under larval brachyurization development and immune challenge stress by Vibrio parahaemolyticus injection in adult crabs were evaluated. EsTUBA cDNA was 1,695 base pairs (bp) with an open-reading frame of 1,356 bp encoding 451 amino acids (49.95 kDa) and containing GTPase domain and C-terminal Tubulin domain. Sequence alignment showed that EsTUBA shared 95-98% identity and exactly similar structural features with its counterparts reported in other animals. Phylogenetic analysis indicated that EsTUBA clustered into one group together with Tubulins from other crustaceans. Sequence alignment, structure comparison and bioinformatic analyses revealed that EsTUBA is member of the Tubulin family. EsTUBA was fluctuant expressed in all tested larval development stages and various organs, and the levels of EsTUBA mRNA expressed in hepatopancreas, gill and intestine, are significantly expression fluctuated and induced after bacteria V. parahaemolyticus injection, while in heart, is almost consistently expressed at 0, 6, 12, 24, 36 and 48 h post-injection compared to control. The molecular cloning of EsTUBA gene is important for further study on the function of this gene.

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