alexa Identification of Salt-responsive Biosynthesis Genes in Rice via Microarray Analysis
ISSN: 2375-4338

Rice Research: Open Access
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Research Article

Identification of Salt-responsive Biosynthesis Genes in Rice via Microarray Analysis

Chang-Kug Kim 1#,Hyeon-So Ji 1#,Hak-Bum Kim1,Doh-Won Yun 2,Gang-Seob Lee1,Ung-Han Yoon1,Tae-Ho Kim1,Dong-Suk Park1,Young-Joo Seol1and Yong-Jae Won3*
1Genomics Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA), Suwon 441-707, Korea
2Planning & Coordination Division, National Academy of Agricultural Science, Suwon 441-707, Republic of Korea
3Rice Research Division, National Institute of Crop Science, Suwon 441-857, Korea
#These authors contributed equally to this work
Corresponding Author : Yong-Jae Won
Rice Research Division
National Institute of Crop Science
Suwon 441-857, Korea
Tel: 82-31-290-6710
Fax: 82-31-290-6730
E-mail: [email protected]
Received June 11, 2013; Accepted July 24, 2013; Published July 26, 2013
Citation: Chang-Kug K, Hyeon-So J, Hak-Bum K, Doh-Won Y, Gang-Seob L, et al. (2013) Identification of Salt-responsive Biosynthesis Genes in Rice via Microarray Analysis. J Rice Res 1:102. doi: 10.4172/jrr.1000102
Copyright: © 2013 Chang-Kug K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

We used a multiple screening process to identify genes involved in salt tolerance–specific biosynthesis and metabolism in rice (Oryza sativa L.). We selected 8,275 salt tolerance–related candidate genes using expression profiles generated across four stages on a microarray containing 135,000 probes (135 K microarray) in Oryza sativa. Using our method, we screened 342 ortholog genes, and 74 pathway genes associated with salt –response–related biosynthesis. Finally, we identified six genes by comparison of pathway-network genes and orthologous genes. The six genes were anchored to the chromosomes of rice to characterize their genetic-map positions and were used to construct the phylogenetic tree. The results were verified by reverse transcription–polymerase chain reaction.

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