In vitro Regeneration of Multipurpose Medicinal Tree Stereospermum Suaveolens-Factors Controlling the In Vitro RegenerationSusmita Shukla*, S.K Shukla and S.K. Mishra
Amity Institute of Biotechnology, Amity University, Sector-125, Noida-201303, India
- Corresponding Author:
- Sushmita Shukla
Amity Institute of Biotechnology, Amity University
Sector-125, Noida-201303, India
E-mail: [email protected]
Received date: November 02, 2012; Accepted date: November 23, 2012; Published date: November 27, 2012
Citation: Shukla S, Shukla SK, Mishra SK (2012) In vitro Regeneration of Multipurpose Medicinal Tree Stereospermum Suaveolens–Factors Controlling the In Vitro Regeneration. J Biotechnol Biomaterial S13:001. doi:10.4172/2155-952X.S13-001
Copyright: © 2012 Shukla S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Stereospermum suaveolens (DC.) is a multipurpose tree endowed with medicinal properties and good timber wood quality. It has analgesic and wound healing property. It is one of the important tree species used in the preparation of ayurvedic formulation known as Dashmula, in which roots of Dashmula tree species are used in preparation of ayurvedic formulations. Wood is employed for construction work, planks and beam, carts, carriages and wagons, furniture, cabinet work and tool handles. The destruction of this plant species is severe and planting of seedlings is almost negligible. Due to low germination and unsuccessful vegetative propagation, in vitro regeneration of Stereospermum suaveolens through micropropagation was studied as a tool for large scale propagation. In the present study, shoots were initiated from the nodal segments of Stereospermum suaveolens further shoot proliferation was achieved on MS medium supplemented with different concentrations of cytokinins and additives.100% root initiation was achieved in the MS medium supplemented with IBA. Different substrates were evaluated during hardening phase. The plants of S. suaveolens grown on coco peat showed 84.6 percent survival and those on soil mixture showed 76.47 percent survival.