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Rice Research: Open Access
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In vitro Screening and Molecular Characterization of a Bacterial BlightResistance Gene in Rice

Dongying Gao* and Lihua Su
Jiangsu Academy of Agricultural Sciences, China
Corresponding Author : Dongying Gao
Center for Applied Genetic Technologies
University of Georgia, USA
E-mail: [email protected]
Received June 17, 2013; Accepted August 02, 2013; Published August 09, 2013
Citation: Gao D, Sun L (2013) In vitro Screening and Molecular Characterization of a Bacterial Blight Resistance Gene in Rice. J Rice Res 1:104. doi: 10.4172/jrr.1000104
Copyright: © 2013 Gao D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Bacterial blight (BB) is one of the most serious bacterial diseases of rice in the world. Since 1986, our laboratory has conducted in vitro screening and application of BB resistance somaclonal mutants for rice breeding. In this paper, we review our nearly 30-year research on BB resistance somaclonal variation, including the development of an in vitro selection system, discovery of BB resistance mutants, evaluation of BB resistance in a somaclonal mutant named HX-3, genetic analysis and molecular mapping of the new BB resistance gene Xa-25. In addition, we also used the new resistance gene to develop BB resistance cultivar and hybrid rice. Our long-term research contributed a novel resistance gene in rice, it also provided an example for new gene creation and discovery using somaclonal variation.

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