In Vitro Test and Molecular Docking of Alkaloid Compound in Marine Sponge Cinachyrella anomala against T47D Cell Cycle
- *Corresponding Author:
- Awik Puji Dyah Nurhayati
Biology Department, Mathematic and Natural Science Faculty
Sepuluh November Institute of Technology Surabaya, Indonesia
Tel: +1-031-596-3857 (extn. 123)
E-mail: [email protected]
Received date February 26, 2015; Accepted date March 28, 2015; Published date March 30, 2015
Citation: Nurhayati APD, Pratiwi R, Wahyuono S, Istriyati, Purnomo H, et al. (2015) In Vitro Test and Molecular Docking of Alkaloid Compound in Marine Sponge Cinachyrella anomala against T47D Cell Cycle. J Marine Sci Res Dev 5:158. doi:10.4172/2155-9910.1000158
Copyright: © 2015 Nurhayati APD, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The compound 1,4,9-triazatricyclo[7,3,1,0]trideca-3,5(13),10-trien-8-ol (SA2014) was isolated from the marine sponge Cinachyrella anomala. In vitro assay for SA2014 compound was found to be able to induce cell-cycle arrest at the sub-G1 and G2/M phases of T47D cancerous cell. A combined dosage between of SA2014 compound and of doxorubicin was able to induce cell-cycle arrest at sub-G1 and G2/M phases. Molecular docking approach showed that SA2014 compound inhibited cdk2 enzyme. The strength of interaction between SA2014 and cdk2 (docking score = -65,43) was more stable than the interaction between doxorubicin and cdk2 (-36,59).