alexa Isolation and Identification of <em>Mycobacterium tuberculosis</em> with Mixed Growth from Positive MGIT 960 Cultures by Re-Decontamination | OMICS International | Abstract
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
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Research Article

Isolation and Identification of Mycobacterium tuberculosis with Mixed Growth from Positive MGIT 960 Cultures by Re-Decontamination

Vasim Ahmad1, Hanif M2*, Chopra KK2, Zeeshan Sidiq2, Kaushal Kumar Dwivedi2 and Divy Shrivastsava1

1School of Life Sciences, Jaipur National University, Jagatpura, Jaipur, Rajasthan, India

2Department of Mycobacteriology, New Delhi Tuberculosis Centre, New Delhi, India

Corresponding Author:
Hanif M
Department of Mycobacteriology
New Delhi Tuberculosis Centre
Jawaharlal Nehru Marg, Delhi Gate
New Delhi, India-110002
Tel: 011-23239056
Fax: 011-23210549
E-mail: [email protected]

Received date: July 26, 2017; Accepted date: August 29, 2017; Published date: September 05, 2017

Citation: Ahmad V, Hanif M, Chopra KK, Sidiq Z, Dwivedi KK, et al. (2017) Isolation and Identification of Mycobacterium tuberculosis with Mixed Growth from Positive MGIT 960 Cultures by Re-Decontamination. J Biotechnol Biomater 7:267. doi:10.4172/2155-952X.1000267

Copyright: © 2017 Ahmad V, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Abstract

Tuberculosis (TB) caused by Mycobacterium tuberculosis has been identified as a remerging infectious disease with public health importance globally. Exploitation of better diagnosis techniques for precise isolation of mycobacteria in clinical specimens is of great importance to improve the diagnosis as part of the global TB control efforts. Contamination of broth cultures of acid-fast bacilli (AFB) by bacterial species other than Mycobacterium species frequently occurs. Many of these contaminated cultures require re-decontamination and re-incubation before the appropriate tests can be performed for identification. This study aimed at the isolation/recovery of M. tuberculosis from contaminated MGIT tubes by making minor modification to the standard protocol and access whether the modification has any impact on recovery rate. Among all confirmed 451 mixed growth positive cultures, 89.57% were culture positive (positive for MTBC), 3.80% were culture negative, 4.65% were culture contaminated and 1.99% were found NTM.  

Keywords

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