Molecular Cloning and In Silico Sequence Analysis of Glycine Betaine Biosynthesis Genes in Bacillus subtilisLawrance Anbu Rajan*, Krishnakumar Vinodhini, Yeragudipati Rajalakshmi and Vetrivel Umashankar
School of Biosciences, Department of Bioinformatics, SRM University, Ramapuram, Chennai, Tamil Nadu, India
- Corresponding Author:
- L. Anbu Rajan
School of Biosciences, Department of Bioinformatics
SRM University, Ramapuram, Chennai, Tamil Nadu, India
Received date: October 15, 2010; Accepted date: February 24, 2011; Published date: March 21, 2011
Citation: Rajan LA, Vinodhini K, Rajalakshmi Y, Umashankar V (2011) Molecular Cloning and In Silico Sequence Analysis of Glycine Betaine Biosynthesis Genes in Bacillus subtilis. J Biotechnol Biomaterial 1:103. doi:10.4172/2155-952X.1000103
Copyright: © 2011 Rajan LA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Glycine betaine (N, N, N-trimethylglycine) is a effective compatible solute, which maintains fluidity of membranes and protects the biological structure of the organisms under stress. In this study, betaine aldehyde dehydrogenase (GbsA) and betaine alcohol dehydrogenase (GbsB) genes encoding for glycine betaine biosynthesis were PCR amplified from genomic DNA of Bacillus subtilis isolated from salted anchovies (Thrissina thryssa) collected from retail fish market of Cochin, Kerala, India. The amplified genes were cloned and nucleotide sequences were determined. The sequencing results showed that GbsA and GbsB genes contain ORF of 1473 bp and 1182 bp long, encoding 474 and 266 amino acids respectively (GenBank accession nos. FJ823257 and FJ823258). In silico sequence analysis revealed that the GbsA and GbsB sequences of B. subtilis were conserved in many eubacteria.