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Obtaining of SiO2 Nanostructured Materials for Local Drug Delivery of Methotrexate.
López T1,2,5*, Alvarez M2, Arroyo S2,6, Sánchez A3, Rembao D5 and López R21Depto. de Atención a la Salud, Universidad Autónoma Metropolitana-Xochimilco, Calz. Del Hueso 1100, Col. Villa Quietud, 04960, Tlalpan, México, DF
2Laboratorio de Nanotecnología, Instituto Nacional de Neurología y Neurocirugía “MVS”. Insurgentes Sur 3877, Col. La Fama, C. P. 14269, Tlalpan, México
3Laboratorio de Neuropatología, Instituto Nacional de Neurología y Neurocirugía “MVS”. Insurgentes Sur 3877, Col. La Fama, C. P. 14269, Tlalpan, México
4Laboratorio de Patología Experimental, Instituto Nacional de Neurología y Neurocirugía “MVS”. Insurgentes Sur 3877, Col. La Fama, C. P. 14269, Tlalpan, México
5Department of Chemical and Biomolecular Engineering, Tulane University, New Orleans, LA 70118 USA
6Escuela Superior de Medicina. Instituto Politécnico Nacional. Plan de San Luis y Díaz Mirón s/n, Col. Casco de Santo Tomas, Delegación Miguel Hidalgo, C.P. 11340, México, DF
- Corresponding Author:
- Tessy López, PhD
Insurgentes Sur 3877, Col La Fama, CP 14269, Tlalpan, México, DF
Tel: +52 55 56063822 ext 5034
Fax: +52 55 5528 8036
E-mail: tessy3@prodigy.net.mx
Received date: June 03, 2010; Accepted date: November 13, 2011; Published date: November 13, 2011
Citation: López T, Alvarez M, Arroyo S, Sánchez A, Rembao D, et al. (2011) Obtaining of SiO2 Nanostructured Materials for Local Drug Delivery of Methotrexate. J Biotechnol Biomaterial S4:001. doi:10.4172/2155-952X.S4-001
Copyright: © 2011 López T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Local delivery of drugs has become an alternative to overcome several side effects of conventional drug administration methods. Because of their novel properties, nanomaterials for controlled drug release have shown enormous potential in the treatment of cancer. In the present work a chemotherapeutic agent, methotrexate (MTX) was encapsulated into a sol-gel SiO2 matrix, as a reservoir for local controlled release in the treatment of a solid brain tumor like Glioblastoma Multiforme (GBM) in an animal model. In order to study interactions between the drug and the SiO2, several physicochemical characterization techniques were carried out. FTIR spectroscopy showed evidence drug incorporation into the silica matrix as the presence of bands at 1504 cm-1, 1604 cm-1 and 1637 cm-1 reveals. This is supported by UV-Vis results, since silica spectrum acquired new features. The materials are mesoporous with a mean pore diameter about 78 Å. In vitro drug release was performed by UV-Vis spectroscopy following the main absorption band of MTX at 348 nm. Drug release was followed and monitored by 16 days. For in vivo evaluation GBM model was performed in Wistar rats once the tumor was developed a reservoir was placed by stereotactic surgery. After three months of tumor inoculation animals treated with MTX-SiO2 survived while in those untreated survival did not exceed 30 days.
