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One-Step Electrodeposition of Gold-Graphene Nanocomposite for Construction of Cholesterol Biosensor | OMICS International | Abstract
ISSN: 2090-4967

Biosensors Journal
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Review Article

One-Step Electrodeposition of Gold-Graphene Nanocomposite for Construction of Cholesterol Biosensor

Hongfang Zhang*, Pengli Li and Mengmeng Wu

College of Chemistry and Materials Science, Northwest University, China

Corresponding Author:
Zhang H
Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecular Chemistry
College of Chemistry and Materials Science
Northwest University
Xi'an 710069, P. R. China
Tel: +1 425-822-8266
E-mail: [email protected]

Received Date: August 25, 2015; Accepted Date: September 17, 2015; Published Date: September 19, 2015

Citation: Zhang H, Li P, Wu M (2015) One-Step Electrodeposition of Gold-Graphene Nanocomposite for Construction of Cholesterol Biosensor. Biosens J 4:128. doi:10.4172/2090-4967.1000128

Copyright: © 2015 Zhang H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The determination of cholesterol is increasingly important for the fact that high serum cholesterol level was related with many diseases. In this work, a new cholesterol biosensor was developed based on the gold-graphene nanocomposite material prepared by one-step electrodeposition. The nanomaterial formed on a glassy carbon electrode was proved by scanning electron microscope and the electrochemical behavior of the modified electrode as well as the cyclic voltametry and electrochemical impedance spectroscopy of the ferricyanide/ferrocyanide couple. After the immobilizing of cholesterol oxidase cross-linked with chitosan, a simple cholesterol biosensor was prepared. The biosensor responded linearly to cholesterol in the concentration range of 0.25 to 5.0 mM with a detection limit of 50 μM. The sensor has the advantages of easy preparation, short response time and good selectivity. Thus, it could be used to determine the free cholesterol in serum.

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