Optimisation of the Chitinase Production by Serratia Marcescens DSM 30121T and Biological Control of LocustsBenine Mohamed Lamine1, Bendaha Mohammed Lamine1 and Abbouni Bouziane2*
- Corresponding Author:
- Prof. Dr. Abbouni Bouziane
Laboratory for the synthesis of the Environmental Information
Department of Biology, Faculty of Science
Sidi Bel Abbés University, 22000-Algeria
E-mail: [email protected]
Received date: March 12, 2012; Accepted date: March 27, 2012; Published date: March 29, 2012
Citation: Lamine BM, Lamine BM, Bouziane A (2012) Optimisation of the Chitinase Production by Serratia Marcescens DSM 30121T and Biological Control of Locusts. J Biotechnol Biomaterial 2:133. doi:10.4172/2155-952X.1000133
Copyright: © 2012 Lamine BM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Serratia marcescens DSM 30121T was tested for their chitinolytic activity on colloidal chitin agar. The production of the chitinase by Serratia marcescens DSM 30121Twas optimized by using of different parameters such as temperature, pH-value, colloidal chitin concentration and incubation time. The obtained results showed that the maximum chitinase production (0.556 U/ml) was observed at the pH-value of 6, at 30°C. Similarly, the recorded maximum chitinase production (0.368 U/ml) was obtained in the presence of 2% of colloidal chitin concentration in the culture medium after 144 h incubation time.
Furthermore, Serratia marcescens DSM 30121 T showed clear zones of hydrolysis of colloidal chitin on agar. Therefore, the present study showed that Serratia marcescens DSM 30121 T is good source for chitinase production. Moreover, the strain Serratia marcescens DSM 30121 T can be used for biological control of locusts.