Optimization of Lipase Catalyzed Synthesis of Nonyl Caprylate using Response Surface Methodology (RSM)Syamsul Kamar MW1*, Salina MR1, Siti Salhah O1, Hanina MN1 and Mohd Basyaruddin AR2
- Corresponding Author:
- Syamsul Kamar MW
Faculty of Science and Technology
Universiti Sains Islam Malaysia (USIM)
Bandar Baru Nilai, 71800 Nilai, Negeri Sembilan, Malaysia
E-mail: [email protected]
Received date: April 19, 2011; Accepted date: June 22, 2011; Published date: June 24, 2011
Citation: Syamsul Kamar MW, Salina MR, Siti Salhah O, Hanina MN, Mohd Basyaruddin AR (2011) Optimization of Lipase Catalyzed Synthesis of Nonyl Caprylate using Response Surface Methodology (RSM). J Biotechnol Biomaterial 1:106. doi:10.4172/2155-952X.1000106
Copyright: © 2011 Syamsul Kamar MW, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Nonyl caprylate, a citrus and rose flavor is short-chain ester with fruity notes is widely used in food, cosmetic and pharmaceutical industries. Traditionally, flavor esters are produced by chemical method or extracted from natural sources. However, with the steadily growing demand for natural flavor compounds, the biosynthesis of such esters by lipase under mild conditions has been receiving much attention for producing these valuable products. In this study, enzymatic synthesis of nonyl caprylate in solvent free system, was successfully optimized via Response Surface Method (RSM) based on 5-level, 4-variable of Central Composite RotaTable Design (CCRD). The parameters were reaction time (3-8 hours); reaction temperature (30-50°C); amount of enzyme (10-20 %, w/w) and shaking speed (100-200 rpm). The optimum condition derived via RSM for the reaction was reaction time of 6.6 hours, reaction temperature of 30.08ºC, enzyme amount of 20 % (w/w) and shaking speed of 128.7 rpm. The actual experimental yield was 90.91% under the optimum condition, which compared well with the maximum predicted value of 91.33%. Comparison of predicted and experimental values reveals good correspondence between them, implying that empirical models derived from RSM can be used to adequately describe the relationship between the factors and response in the synthesis of Nonyl Caprylate.