alexa Production of Proteases by Genetically Improved Bacillus subtilis for Enhanced Skin Penetration of Antibacterial Topical Formulation
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

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Research Article

Production of Proteases by Genetically Improved Bacillus subtilis for Enhanced Skin Penetration of Antibacterial Topical Formulation

Faisal Shahzad1, Sohail Ahmad2, Habib Ahmad1, Qazi Abdur Rashid2, Nadia Parveen3, Fatima Javed3, Rida Khan4, Muhammad Zeeshan Ashraf3 and Faiza Naseer3*

1Department of Genetics, Hazara University, Mansehra, Pakistan

2Department of Biochemistry, Hazara University, Mansehra, Pakistan

3Department of Pharmacy, Government College, University Faisalabad, Pakistan

4Department of Physiology and Pharmacology, Agriculture University, Faisalabad, Pakistan

Corresponding Author:
Faiza Naseer
Department of Pharmacy, Government College
University Faisalabad, Pakistan
E-mail: [email protected]

Received date:: June 06, 2015; Accepted date:: July 07, 2015; Published date:: July 14, 2015

Citation: Shahzad F, Ahmad S, Ahmad H, Rashid QA, Parveen N, et al. (2015) Production of Proteases by Genetically Improved Bacillus subtilis for Enhanced Skin Penetration of Antibacterial Topical Formulation. J Biotechnol Biomater 5:186. doi:10.4172/2155-952X.1000186

Copyright: © 2015 Shahzad F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The present study was designed for the production, isolation and extraction of protease enzyme from Bacillus subtilis and to investigate the role of protease enzyme mixed with standard antibacterial formulation, i.e. Silver Sulphadiazine 1% w/w cream in burnt abscessed wounds to improve and speed up the healing process. 18 male rabbits weighing between 1.8-2 kg were divided into 3 groups. A burnt abscessed wound model was created on the back of all the rabbits and pathogenic Streptococci introduced into the wound. Then the burnt abscessed areas in 1st, 2nd and 3rd groups were applied daily with cold cream (control), Silver Sulphadiazine 1% w/w cream and Silver Sulphadiazine 1% + Protease w/w cream respectively. The wounds were examined on a daily basis for the healing process; sizes were measured and recorded in the form of photographs on 2nd, 5th, 10th and 14th day. The rabbits were anesthetized and skin tissue samples of each group were collected for histo-pathological examinations. Significant improvement in wound healing with Silver Sulfadiazine 1% w/w and Proteases cream was noticed as compared to Silver Sulphadiazine 1% w/w cream and cold cream (control). It was concluded that application of Silver Sulfadiazine 1% w/w and Proteases was appreciably useful in healing of burnt and abscessed skin wounds in animal model (rabbit) and antibacterial formulation Silver Sulphadiazine mixed protease enzyme can be exploited in pharmaceutical field as an effective promoter of wound healing. The studies can be extended further for formulation stability and clinical trials on human.

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