Properties of Alkaline Protease C<sub>45</sub> Produced by Alkaliphilic <em>Bacillus</em> Sp. Isolated from Chitu, Ethiopian Soda Lake | OMICS International | Abstract
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Properties of Alkaline Protease C45 Produced by Alkaliphilic Bacillus Sp. Isolated from Chitu, Ethiopian Soda Lake

Gizachew Haile1* and Amare Gessesse2

1Jimma University, College of agriculture and veterinary medicine, Biotechnology program, Jimma, Ethiopia

2Addis Ababa University Biotechnology grogram, Addis Ababa, Ethiopia

Corresponding Author:
Gizachew Haile
Addis Ababa University Biotechnology grogram
Addis Ababa, Ethiopia
E-mail: [email protected]

Received date: March 15, 2012; Accepted date: April 14, 2012; Published date: April 16, 2012

Citation: Haile G, Gessesse A (2012) Properties of Alkaline Protease C45 Produced by Alkaliphilic Bacillus Sp. Isolated from Chitu, Ethiopian Soda Lake. J Biotechnol Biomater 2:136. doi:10.4172/2155-952X.1000136

Copyright: © 2012 Haile G, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


A total of 240 alkaliphilic bacteria were isolated from samples collected from alkaline soda lakes of Ethiopia and were screened for the production of alkaline proteases. Of these, 30% were protease positive indicating the abundance of protease producing microorganisms in these habitats. The Bacillus sp. designated as C45 that grow in solid state medium was selected based on the property of the enzyme for further study. The protease produced by the Bacillus sp. was active in the pH range of 6.5-11.5, with optimum activity at pH 8-9; and stable at alkaline pH. The optimum temperature for activity was 40°C and 50°C in absence and presence of 5 mM of Ca+2, respectively. The enzyme displayed appreciable activity and stability at low temperature. These properties suggest that protease C45 could find potential application for dehairing and detergent at moderate temperature. When protease C45 was added to raw hide it enabled dehairing, suggesting the potential usefulness of the enzyme in the leather industry.

Recommended Conferences

2nd International Conference on Biotechnology and Healthcare

Auckland, New Zealand
Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Share This Page