Protective Effects of Tannic Acid against Methomyl-Induced-Oxidative StressOmali Y El-Khawaga*
Faculty of Community, King Khalid University, Rejal Almaa, Kingdom of Saudi Arabia
- Corresponding Author:
- Omali Y El-Khawaga
Faculty of Community, King Khalid University
Rejal Almaa, Kingdom of Saudi Arabia
E-mail: [email protected]
Received date: January 02, 2012; Accepted date: February 09, 2012; Published date: February 11, 2012
Citation: El-Khawaga OY (2012) Protective Effects of Tannic Acid against Methomyl- Induced-Oxidative Stress. J Biotechnol Biomaterial 2:127. doi:10.4172/2155-952X.1000127
Copyright: © 2012 El-Khawaga OY. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Methomyl carbamate is a pesticide widely used in the control of insects. The present work aims at studying the effect of tannic acid on the antioxidant system of methomyl-treated-mice. Swiss albino mice were intraperitoneally administered a single dose of methomyl (7 mg/Kg b.wt). Mice of another group were injected with tannic acid (20 mg/ Kg b.wt.) 3 hrs before methomyl intoxication. After 24 hours, methomyl exposure resulted in significant increase in lactic dehydrogenase activity (LDH). The antioxidant capacity of hepatic cells in terms of the activities of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione-S-transferase (GST) and glutathione (GSH) content was diminished. It appears that methomyl exerts its toxic effect via peroxidative damage to hepatic, renal and splenic cell membranes. Also, methomyl induced DNA damage in these organs as detected by alkaline filter elution technique. The distribution of methomyl in different organs of mice was detected by HPLC. Tannic acid administration prior to methomyl injection produced pronounced protective action against methomyl effects. It is observed that tannic acid enhances the endogenous antioxidant capacity of the cells by increasing the activities of SOD, CAT, GR and GST as well as increasing GSH content. The activity of LDH was decreased in liver and the damage of DNA was suppressed comparable to controls. In conclusion, this study concludes that tannic acid has a potential in mitigating most of the adverse effects induced by methomyl toxicity.