Real-time PCR: Transforming Modern Diagnostics
*Corresponding Author:Received Date: Nov 01, 2025 / Accepted Date: Dec 01, 2025 / Published Date: Dec 01, 2025
Citation: Dimitrov DI (2025) Real-time PCR: Transforming Modern Diagnostics. awbd 14: 330.DOI: 10.4172/2167-7719.1000330
Copyright: © 2025 Dr. Ivan Dimitrov This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
Abstract
Real-time PCR technology is rapidly advancing diagnostics across diverse fields. New multiplex assays detect multiple diarrheal pathogens, human adenovirus, and fungal infections, offering superior sensitivity and specificity for timely clinical decisions. The technology also tackles antibiotic resistance by identifying resistance genes and aids malaria diagnosis with low parasite loads. Beyond clinical settings, it enhances water quality monitoring for Legionella, ensures food safety by detecting major foodborne pathogens, and enables early diagnosis of plant diseases. Real-time PCR proves crucial for complex challenges like tuberculosis detection and rifampicin resistance, and for rapid identification of zoonotic agents such as Brucella and Coxiella burnetii, thus providing comprehensive and efficient solutions for public and environmental health.

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