Semi-interpenetrated Hydrogels Composed of PVA and Hyaluronan or Chondroitin Sulphate: Chemico-Physical and Biological CharacterizationAntonella D Agostino, Annalisa La Gatta, Teresa Busico, Mario De Rosa and Chiara Schiraldi*
Department of Experimental Medicine, Section of Biotechnology and Molecular Biology, Second University of Naples, Via De Crecchio No 7, 80138 Naples, Italy
- Corresponding Author:
- Prof. Chiara Schiraldi
Department of Experimental Medicine
Section of Biotechnology and Molecular biology
via De Crecchio No 7, 80138 Naples, Italy
E-mail: [email protected]
Received date: April 26, 2012; Accepted date: June 23, 2012; Published date: June 25, 2012
Citation: Agostino AD, Gatta AL, Busico T, Rosa MD, Schiraldi C (2012) Semi-interpenetrated Hydrogels Composed of PVA and Hyaluronan or Chondroitin Sulphate: Chemico-Physical and Biological Characterization. J Biotechnol Biomater 2:140. doi:10.4172/2155-952X.1000140
Copyright: © 2012 Agostino AD, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Physical hydrogels based on poly (vinyl alcohol) (PVA) were synthesized in the presence of natural polysaccharides, either hyaluronan or Chondroitin sulphate, in order to obtain versatile biomaterials with enhanced performances. The physical network is obtained by the freeze thawing technique, a simple method widely used for structuring PVA blends. The chemico-physical characterization of resulting materials consisted of swelling studies and mechanical analysis. Furthermore the release of embedded polysaccharides from the network was evaluated to improve understanding of the strength of hydrogen bonding between the different polymeric chains, and the effect of the sulphate groups on the interaction promoting network formation and stability. Biological response in terms of cytotoxicity, adhesion and cell vitality of murine fibroblast and human keratinocytes showed that the addition of glycosaminoglycans to a PVA polymer leads to a biomaterial with potential applications in biomedical fields.