Thick Juice-Based Production of Amino Acids and Putrescine by Corynebacterium glutamicumTobias M Meiswinkel, Steffen N Lindner and Volker F Wendisch*
Chair of Genetics of Prokaryotes, Faculty of Biology and CeBiTec, Bielefeld University, Germany
- Corresponding Author:
- F Wendisch
Chair of Genetics of Prokaryotes,Faculty of Biology and CeBiTec
Bielefeld University, Universitätsstr 25, D-33615 Bielefeld, Germany
E-mail: [email protected]
Received date: August 19, 2014; Accepted date: October 06, 2014; Published date: November 15, 2014
Citation: Meiswinkel TM, Lindner SN, Wendisch VF (2014) Thick Juice-Based Production of Amino Acids and Putrescine by Corynebacterium glutamicum. J Biotechnol Biomater 4:167. doi:10.4172/2155-952X.1000167
Copyright: 2014 Meiswinkel TM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Thick juice (also regarded as syrup) is an intermediate product of sugar processing. It is cheaper than processed sugar and is mainly composed of sucrose. Sucrose is a preferred carbon source of Corynebacterium glutamicum, a workhorse of biotechnology used for million-ton-scale amino acid production. Here, it is shown for C. glutamicum that sugar beet thick juice led to higher growth rates and faster carbon source consumption than pure sucrose. Comparative DNA microarray analysis revealed differential expression of genes for butyrate and citrate catabolism and of NAD and biotin biosynthesis suggesting provision of these compounds by the thick juice. Thick juice was also shown to be superior to sucrose in production of the amino acids L-lysine, L-glutamate and L-arginine as well as of the diamine putrescine since higher volumetric productivities than with pure sucrose could be achieved. Taken together, sugar beet thick juice was shown to be a carbon source for growth and amino acid and diamine production of C. glutamicum superior to pure sucrose.