alexa Thidiazuron Induces High Frequency Shoot Regeneration i
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Thidiazuron Induces High Frequency Shoot Regeneration in Leaf and Petiole Explants of Cabbage (Brassica Oleracea L. Var. Capitata)

Geetika Gambhir* and Srivastava DK

Department of Biotechnology, Dr. Y.S. Parmar University of Horticulture and Forestry, Himachal Pradesh, India

Corresponding Author:
Geetika Gambhir
Department of Biotechnology
Dr. Y.S. Parmar University of Horticulture and Forestry
Solan-173230, Himachal Pradesh, India
E-mail: [email protected][email protected]

Received date: January 16, 2015; Accepted date: March 13, 2015; Published date: March 20, 2015

Citation: Gambhir G, Srivastava DK (2015) Thidiazuron Induces High Frequency Shoot Regeneration in Leaf and Petiole Explants of Cabbage (Brassica Oleracea L. Var. Capitata). J Biotechnol Biomater 5:172. doi:10.4172/2155-952X.1000172

Copyright: © 2015 Gambhir G, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

We have studied the effect of thidiazuron on shoot regeneration from leaf and petiole explants of cabbage (Brassica oleracea L. var. capitata cv. Pride of India). Different concentrations and combinations of thidiazuron (alone), thidiazuron+ Adenine, thidiazuron+ NAA and thidiazuron+ IAA were used in MS medium to optimize in vitro shoot regeneration from leaf and petiole explants of cabbage (Brassica Oleracea L. var. capitata cv. Pride of India). Leaf and petiole explants procured from 20-25 days old glasshouse grown seedlings of cabbage. Out of 40 combinations and concentrations tried separately for leaf and petiole explants, the high frequency shoot regeneration from leaf (91.11%) and petiole (88.88%) explant was obtained on MS medium supplemented with0.220mg/l thidiazuron + 0.02 mg/l NAA and 0.330 mg/l thidiazuron + 0.02 mg/l NAA respectively. High percentage root regeneration (100%) in in vitro developed shoots was obtained on MS medium supplemented with 0.10mg/l NAA. The regenerated complete plantlets were transferred to pots containing sterilized coco peat and acclimatized. A high efficiency and reliable plant regeneration protocol has been developed using thidiazuron. This regenerated protocol would be useful for genetic transformation studies in cabbage.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords