Toxicity Effects of the Environmental Hormone 4-Tert-Octylphenol in Zebrafish (Danio Rerio)
- *Corresponding Author:
- Shao-Yang Hu
Department of Biological Science and Technology
National Pingtung University of Science and Technology
No. 1, Hseufu Road, Neipu, Pingtung 912, Taiwan
E-mail: [email protected]
Received date: October 09, 2015; Accepted date: January 21, 2016; Published date: January 27, 2016
Citation: Saputra F, Yen CH, Hsieh CY, Ou TY, Risjani Y, et al. (2016) Toxicity Effects of the Environmental Hormone 4-Tert-Octylphenol in Zebrafish (Danio Rerio). J Marine Sci Res Dev 6:180. doi:10.4172/2155-9910.1000180
Copyright: © 2016 Saputra F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
4-tert-octylphenol (4-t-OP), an environmental exogenous estrogen is produced by microbial degradation of alkylphenol polyethoxylates (APEOs). Although it is well known that 4-t-OP can cause the feminization of male, sterility and deficiency of gonad development of aquatic animals by disrupting the endocrine reproductive signaling, less is known about the effects of 4-t-OP on embryonic development. Moreover, the presence of 4-t-OP were detected in umbilical cord blood samples of newborns suggesting infants during development may expose to the risk of 4-t-OP contaminant, hence to investigate the effect of 4-t-OP on physiological function during embryonic development is necessary. In the present study, zebrafish embryos exposed to 4-t-OP were used to evaluate the toxicity of 4-t-OP. The 50% lethal dose (LD50) for wild type zebrafish embryos exposure to 4-t-OP for 3 days is approximately 1.0 μM, and a high ratio of cardiovascular defects were showed in survival embryos. To observe the cardiovascular defects more efficiently, Tg (fil-1: EGFP) zebrafish embryos was used in 4-t-OP exposure treatment. Following exposure Tg (fil-1: EGFP) zebrafish embryos to 4-t-OP at 1.0 μM for 4 days, a highly proportion of defects revealed in cardiovascular system, including pericardical edema, irregular shape or incomplete looping of ventricle and atrium, the absence of intersegmental vessel in the tail of notochord, unformed parachordal vessel and kinks in the caudal vein. The phenotype of cardiovascular defects was accompanied by reduced heart rate and impaired blood circulation. The mRNA expression levels of transcription factors, which are critical for zebrafish heart chamber formation and blood vessel development, were analyzed by RT-PCR. The results showed that the presence of 4-t-OP significantly induce expression level of ERα and ERβ2, and caused cardiovascular defects by suppressing transcription factor Nkx2.7, Hand2, Tbx2a, Tbx2b, Tbx5a, FGF1a, GATA-4, -5 and -6 in zebrafish. The present study suggests that 4-t-OP affects the cardiovascular development in zebrafish and elucidated that early life exposure to 4-t-OP potentially may take a risk of impaired cardiovascular function.