Universal Bioanalyte Signal Amplification for Electrochemical Biosensor
Guanine Inc., 825 N. 300 W. Suite C325, Salt Lake City, UT 84103, USA
- Corresponding Author:
- Neil Gordon
Guanine Inc., 825 N. 300 W. Suite
C325, Salt Lake City, UT 84103, USA
E-mail: [email protected]
Received date: November 25, 2015; Accepted date: January 30, 2016; Published date: February 03, 2016
Citation: Gordon N (2016) Universal Bioanalyte Signal Amplification for Electrochemical Biosensor. Biosens J 4:135. doi:10.4172/2090-4967.1000135
Copyright: © 2016 Gordon N. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A rapid, simple, and inexpensive electrochemical biosensor with guanine-conjugated microbead tags can achieve the detection limits of PCR and culturing. Guanine is a redox species that functions as an electrochemical tag for bioanalyte detection. Microbeads conjugated with guanine-rich oligonucleotides deliver millions of electrochemical tags per bioanalyte. When guanine-conjugated microbeads are used in a sandwich assay with magnetic beads and matched ligand pairs, extremely low levels of diverse bioanalytes can be electrochemically measured including microorganisms, proteins and nucleic acids.