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Distribution Of Collagen Type I And III And Matrix Metalloproteinase-1 Expression In Cases With Clinico-pathological Diagnosis Of Intestinal Tuberculosis | 3554
ISSN: 2161-069X

Journal of Gastrointestinal & Digestive System
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Distribution of collagen type I and III and matrix metalloproteinase-1 expression in cases with clinico-pathological diagnosis of intestinal tuberculosis

2nd International Conference on Gastroenterology & Urology

Minakshi Gulia

AcceptedAbstracts: J Gastrointest Dig Syst

DOI: 10.4172/2161-069X.S1.019

Abstract
Background: India, being a developing country, bears an enormous disease burden of tuberculosis (TB). Average prevalence of all forms of tuberculosis in India is estimated to be 5.05 per 1000 and tuberculosis of GI tract remains the sixth most frequent form of extra-pulmonary TB. Mycobacterium tuberculosis (TB) destroys normal Collagen in lung tissues causing cavitation and this immunopathology is mediated in part by Matrix Metalloproteinase-1 (MMP-1). MMP-1 has also been implicated in the pathogenesis of Inflammatory Bowel Disease, especially Crohn?s disease. There are no studies, however, regarding the role of MMPs-1 in intestinal TB. A cross-sectional study was carried out in a tertiary care center to study the distribution of Collagen type I & III and expression of MMP-1 in intestinal tuberculosis. Materials & methods: In 30 cases of intestinal TB, lesional area (ulcer/ stricture/ perforation with granulomas) and their resected ends (relatively normal, controls), collagen distribution was studied using Picrosirius red staining. MMP-1 expression was determined using Streptavidin biotin method of immunostaining. Results: Total percent collagen was increased in the submucosa (p=0.005) in lesional areas along with a disarray of collagen fibrils. Collagen type I & III was observed at the periphery of granulomas, leading to encapsulation. Confluent granulomas, however, showed reduced deposition of collagen. MMP-1 expression was increased in the cells of the granulomas (p<0.01), most of them showing moderate to intense immunostaining. Fibroblasts, giant cells, epithelioid cells, macrophages and inflammatory cells had an increased MMP-1 expression. Cases with perforation showed an inverse correlation between collagen distribution & MMP-1 expression, suggesting the role of MMP-1 in degradation of collagen in these areas. Conclusion: Intestinal TB shows an increased MMP-1 expression and distribution of Collagen type I & III I the submucosa. Encapsulation of the granulomas occurs, possibly in an attempt to establish a latent infection and there is disarray in the collagen fibrils deposition. A larger sample size could possibly establish an inverse correlation between the two.
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