Reach Us +44-176-4910199
Immunohistochemical Study Of The Stem Cell Marker Foxl1 In Bile Ductular Proliferation And Liver Cell Regeneration In Liver Biopsies From Infants With Cholestasis | 99213
ISSN: 2161-069X

Journal of Gastrointestinal & Digestive System
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Immunohistochemical study of the stem cell marker Foxl1 in bile ductular proliferation and liver cell regeneration in liver biopsies from infants with cholestasis

Joint Event on 13th International Conference on Pediatric Gastroenterology Hepatology & Nutrition & 3rd International Conference on Digestive and Metabolic Diseases

Basma Elhaddad, Dina Abdalla and Mona Abdel-Hadi

Alexandria University, Egypt

Posters & Accepted Abstracts: J Gastrointest Dig Syst

DOI: 10.4172/2161-069X-C7-083

Liver cell regeneration takes place through mature hepatocytes. However, in cases of chronic or severe injury, an alternative pathway takes place through proliferation of hepatic progenitor cells (HPCs). The aim of the study was to investigate the distribution and number of Foxl1 positive HPCs in the liver of infants with different cholestatic diseases. Therefore, immunohistochemistry using Foxl1 antibody (ab190226) was conducted on 53 formalin fixed paraffin embedded blocks of liver biopsies from infants with neonatal cholestasis with the following diagnoses: 30 cases of extra hepatic biliary atresia (EHBA), 11 cases of paucity of intrahepatic bile ducts (PIBD), eight cases of idiopathic neonatal giant cell hepatitis (NGCH) and four cases of galactosemia. Trichrome staining was done to assess the stage of fibrosis according to a previously published modified scoring system. Foxl1 positive cells were seen in the periportal area and their numbers were much higher in liver biopsies obtained from infants with EHBA compared to the other diagnoses. Significant positive correlations were found between the number of HPCs and stage of fibrosis, degree of ductular proliferation, the presence of portal tract neutrophils as well as higher levels of serum gamma glutamyl transferase. In conclusion, HPCs are markedly activated in EHBA and their activation might be the reason for the associated ductular proliferation and fibrosis. However, the type of inflammatory infiltrate might play a role in HPCs activation as well. This may help to guide further research on animal models to design HPC-based anti-fibrotic therapies for cholestatic liver diseases.

Basma Elhaddad is a Faculty Member (Demonstrator) at the Pathology Department, Faculty of Medicine, Alexandria University, Egypt. She graduated from the Faculty of Medicine, Alexandria University in 2010 (MBBCh).She is pursuing Master’s Degree student with a thesis in the Pathology of Gastrointestinal tract and liver “Master of Basic Medical Sciences in Pathology (MSc.); GPA: Excellent with Honour”

E-mail: [email protected]