Journal of Gastrointestinal & Digestive System
Open Access

Abstract

Objective: Influence of CD133 + expression on patients? survival and resistance of CD133 + cells to anti-tumor agents were studied in gastric cancer (GC). Methods & Results: As revealed by Western blot, the lower CD133 + group had a significantly better survival as compared to the higher CD133 + group (P=0.014). From CD133 + subpopulation (28.0% ? 2.0%) for KATO-III cells, the highest content ratio among four measured cells lines and cancerous ascites from GC patient, could be enriched following the immunomagnetic separation (IMS) (91.0% ? 3.0%) and then at 1 wk of floating culture (95.0% ? 2.0%). The population doubling time was 21.0 ? 3.0 h vs. 40.0 ? 8.0 h in CD133 + and CD133 - cells by CCK-8 assay; respectively (P<0.05). As shown, a single CD133 + cell was capable of generating new cell colony and the tumorigenicity rate in nude mice was 100.0% (5/5) for CD133 + clonal spheres or for CD133 + cells, but 0.0% (0/5) for CD133 - cells. The significantly higher mRNA level of Oct-4, Sox-2, Musashi-1, and ABCG2 in CD133 + clonal spheres could be identified comparing to that in either CD133 + cells or in CD133 - cells. Under the treatment of 5-FU, cisplatin or VP-16, CD133 + cells had significantly lower suppression rates of cell growth in comparison with those in CD133 - cells while lower level of Bcl-2 mRNA and higher level of Bax mRNA were simultaneously found in CD133 + cells comparing to that in CD133 - cells (P<0.05). Conclusions: The patients with lower CD133 + expression had a better survival. Enriched CD133 + cells in clonal sphere with higher mRNA level of Oct-4, Sox-2, Musashi-1, and ABCG2 shared the ability to be self-renewable, proliferative, tumorigenic and resistant to anti-tumor agent as probably regulated by genetic products of Bcl-2 and Bax.

Biography

Ji-wei Yu has completed her M.D. at the age of 25 years from Shanghai Jiao-tong University School of Medicine and postdoctoral studies for Ph.D. from Shanghai Jiao-tong University School of Medicine. She is the Director of 1 st Department of General Surgery. She has published more than 50 papers in reputed journals and is serving as an editorial board member of repute.